| Discovery Information |
| Isolation Host | Streptomyces scabiei RL-34 |
| Found By | Patel,Harsh & Patel,Priya |
| Year Found | 2017 |
| Location Found | Owings Mills, MD USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.441938 N, 76.785539 W Map |
| Discovery Notes | Temp 77F; Semi-dry soil found in an empty patch from a garden; around 6 inches deep |
| Naming Notes | HotFries was named after our favorite snack made by Andy Capp's. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 18, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Genome length (bp) | 43699 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCCCT |
| GC Content | 60.9% |
| Sequencing Notes | after position 43651 bp consensus contains string 10 G's, 20% of reads contain 10 or 12 G's |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BI |
| Subcluster | BI2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | A mesa was not formed after spotting 10^0-10^-7 dilutions of the lysate. |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | 2 mm in diameter, cloudy, round, smooth edges |
| Morphotype | Siphoviridae |
| Number of Genes | 57 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH155869 |
| Refseq Number | None yet |
| Published in Paper | Blocker D et al 2018, Genome Announcements: Complete Genome Sequences of Six BI Cluster Streptomyces Bacteriophages, HotFries, Moozy, Rainydai, RavenPuff, Scap1, and SenditCS. |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
9.9x10^9 |
| Pitt Freezer Box# |
49 |
| Pitt Freezer Box Grid# |
F2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
