| Detailed Information for Phage Huphlepuff |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Dalton Schengrund and Anna Anand |
| Year Found | 2021 |
| Location Found | Philadelphia, PA United States |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.946663 N, 75.208668 W Map |
| Discovery Notes | Depth - 1 cm
Moisture - Moist
Located in a flower bed next to the security entrance at Griffith Hall at USciences. Very close to roads (~5 feet away), in a suburban area. Ground is not sloped and no foot traffic should be taking place in the area of collection. |
| Naming Notes | Named after Anna's Hogwarts house, Hufflepuff, but with a bit of zest from the ph- in "phage". |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 26, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 32 |
| Genome length (bp) | 64543 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | GCGCGCAGCGC |
| GC Content | 63.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | S |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of the Sciences (now St. Joseph's Univ) |
| Annotation Status | In GenBank |
| Plaque Notes | Generates three different types of morphologies; small, turbid plaques; large, turbid plaques; and relatively larger plaques that form a bullseye/halo morphology.
The titer collected on 12/2/21 read to be 1.12 x 10^7 pfu |
| Number of Genes | 115 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON637772 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
136 |
| Pitt Freezer Box Grid# |
A10 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
