| Detailed Information for Phage Kenna |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Watole Hamda |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | -22°C |
| GPS Coordinates | 40.446071 N, 79.955902 W Map |
| Discovery Notes | The GPS coordinates of the soil sample location: 40.446071 N, 79.955902 W Weather condition at the time of collection: 20 degree F, overcast, slight winds Weather condition in the last 24 hours: High of 16 degrees F, Min of 7 degrees F Sample was immediately stored and was stored for approx. 30 minutes prior to usage Soil conditions: Hard, Cold, snow on the ground |
| Naming Notes | I decided to name this phage after my little brother, Kenna. His name means "gift" in Oromo, a language commonly spoken in Ethiopia. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 22, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2286 |
| Genome length (bp) | 53215 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CTCGGGGCAT |
| GC Content | 63.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DN |
| Subcluster | DN1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Kenna's plaque morphology consists of plaques that are large (~2.5mm diameter), circular, and clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK279906 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
55 |
| Pitt Freezer Box Grid# |
H5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |