| Detailed Information for Phage KentuckyRacer |
| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Andrew Adams and Ross Rider |
| Year Found | 2023 |
| Location Found | Land Between the Lakes, KY United States |
| Finding Institution | University of Evansville |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.74952 N, 88.07441 W Map |
| Discovery Notes | Sample of soil was collected from an area of high automobile traffic at a dirt racing track in Land Between the Lakes National Recreational Area. The dirt was soft, but not too full of water and a dark brown color. |
| Naming Notes | The name originates from the state the phage was found in as well as the race track it was found near. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 538 |
| Genome length (bp) | 133791 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 12340 bp |
| GC Content | 49.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lytic. |
| Other Cluster Members |
|
| Annotating Institution | University of Evansville |
| Annotation Status | In GenBank |
| Plaque Notes | Phage plaques were always transparent and between 1-2mm in size. Only on extremely low concentration assays of the phage were plaques found smaller than 1mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 250 |
| Number of tRNAs | 44 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ244013 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.4 X 10^9 |
| Pitt Freezer Box# |
173 |
| Pitt Freezer Box Grid# |
E9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
