| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kate Aberger |
| Year Found | 2014 |
| Location Found | New Haven, CT USA |
| Finding Institution | Southern Connecticut State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 42°C |
| GPS Coordinates | 41.3352 N, 72.94176 W Map |
| Discovery Notes | Found in 3 inches deep in very most soil in a compost pile
The temperature was 64 degrees F and it rained over the weekend
Lots of insects could live at the location that I found it
Grass, leaves, tree brances, and dirt surround the site of discovery
Discovered on 9-22-14
Ave capsid width (nm) = 69.81 +/- 3.13
Ave tail length (nm) = 283.58 +/- 7.02 |
| Naming Notes | I named this phage "Lilbit" because it creates tiny plaques with a diameter of 1 mm |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 7, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 419 |
| Genome length (bp) | 65106 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | GCGCGCAGCGC |
| GC Content | 63.4% |
| Sequencing Notes | This phage was identified as being uncommon during a large DOGEMS screening run, then subsequently grown from frozen stock and sequenced. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | S |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Connecticut State Community College Northwestern |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 110 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV876982 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2014SCSUlilbitKA |
| SEA Lysate Titer |
2.5 x 10^10 |
| Date of SEA Lysate Titering |
Oct 21, 2014 |
| Pitt Freezer Box# |
10 |
| Pitt Freezer Box Grid# |
B7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
