| Detailed Information for Phage LittleLaf |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Rachael Broder |
| Year Found | 2016 |
| Location Found | Easton, PA USA |
| Finding Institution | Lafayette College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.697293 N, 75.210184 W Map |
| Discovery Notes | Discovered on the Lafayette College campus near the Kirby Hall of Civil Rights close to the building, on the side of a hill, close to a flower bed. |
| Naming Notes | My phage is very small and I go to Lafayette College so it is called LittleLaf. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 9, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1286 |
| Genome length (bp) | 64834 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | GCGCGCAGCGC |
| GC Content | 63.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | S |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Lafayette College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are very small and almost perfectly circular. |
| Morphotype | Siphoviridae |
| Number of Genes | 112 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH727553 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.67x10^8 |
| Date of SEA Lysate Titering |
Nov 8, 2016 |
| Pitt Freezer Box# |
30 |
| Pitt Freezer Box Grid# |
H5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |