| Detailed Information for Phage Neoh | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Angelee Kang |
| Year Found | 2025 |
| Location Found | Williamsburg, VA US |
| Finding Institution | College of William & Mary |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 21°C |
| GPS Coordinates | 37.269002 N, 76.717805 W Map |
| Discovery Notes | The soil sample was retrieved from moist, organic topsoil surrounded by bricks and a dripping pipe at the base of the William and Mary West Utility Plant. The day of collection was humid with light rain and no sunlight. The drops of the dripping pipe were falling directly underneath onto a broken brick path. Since the bricks were cracked, much of the water seeped through directly into the soil beside it. Additionally, there was a plethora of green vegetation by the spot the soil was collected. About 3 tablespoons of soil was collected from 5.1 centimeters down from the surface. |
| Naming Notes | Neoh was inspired by and named after Neo from the Matrix. In the Matrix, Neo symbolizes emergence, awakening, disrupted systems, and the unexpected. By attacking bacteria and disrupting microbial systems, Neoh perfectly embodies the character of Neo. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 22, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 234 |
| Genome length (bp) | 41894 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCCATGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | G |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewJeilious (None) Neoh (None) Angel (G1) Annihilator (G1) Aroostook (G1) Avrafan (G1) AzulaCat (G1) Barkley26 (G1) BPs (G1) BQuat (G1) BruceB (G1) Camri (G1) CassieYates (G1) Cedasite (G1) Chance64 (G1) Cherrybomb426 (G1) CLED96 (G1) Coleslaw (G1) Crespo (G1) Darionha (G1) DingDing (G1) DMoney (G1) ECartman (G1) Frosty24 (G1) Gideon (G1) GoldenAsh (G1) Gomashi (G1) Grizzly (G1) Halo (G1) Hope (G1) Hotshotbaby7 (G1) Jane (G1) Jolene (G1) Jonghyun (G1) JorRay (G1) Kareem (G1) Kasen3 (G1) Liefie (G1) LouisV14 (G1) Maliketh (G1) Marmie (G1) Mowgli (G1) OctaviousRex (G1) Olga (G1) Paito (G1) Peeb (G1) Periodt (G1) Phish (G1) Phloodle (G1) Phreak (G1) PinkYoshi (G1) Plagueis (G1) Rabbs (G1) Rattrick (G1) Remy19 (G1) Renaissance (G1) Richarlison (G1) Ruriko (G1) Schiebel (G1) ShaboiShabazz (G1) Sizemore (G1) Sneeze (G1) Sweets (G1) Taheera (G1) Terror (G1) TinaBug (G1) TomBrady (G1) Wendigo (G1) ZoMa (G1) Zombie (G1) Avocado (G2) Cambiare (G2) FlagStaff (G2) GodSpeed (G2) Pace1224 (G2) Cloudyecho (G3) MOOREtheMARYer (G3) Pinnie (G3) Jolie2 (G4) Lemuria (G4) Mercurio (G4) Antsirabe (G5) Stargaze (G5) YangYin (G5) |
| Annotating Institution | College of William & Mary |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Tiny, round with a slight streak on the side, translucent, consistently <1 mm in diameter, and no other unique features. All plaques were consistently of one morphology. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
