Discovery Information |
Isolation Host | Streptomyces scabiei RL-34 |
Found By | Chih Shiang Keith Shee, John Varghese |
Year Found | 2018 |
Location Found | Halethorpe, MD USA |
Finding Institution | University of Maryland, Baltimore County |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 28°C |
GPS Coordinates | 39.257417 N, 76.704278 W Map |
Discovery Notes | Plaques were obtained from an enrichment of the soil sample, and then purified using plaque assay 3 times. |
Naming Notes | after Terry Crews. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 24, 2019 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina |
Approximate Shotgun Coverage | 3283 |
Genome length (bp) | 43736 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 9 bases |
Overhang Sequence | CGCCGCCCT |
GC Content | 61.0% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | BI |
Subcluster | BI2 |
Cluster Life Cycle | Lytic |
Other Cluster Members |
|
Annotating Institution | University of Maryland, Baltimore County |
Annotation Status | In GenBank |
Plaque Notes | from the soil near the roots of a white oak tree. |
Morphotype | Siphoviridae |
Number of Genes | 58 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MK686070 |
Refseq Number | None yet |
Published in Paper | Park et al 2022, Microbiology Resource Announcements: Complete Genome Sequences of Streptomyces Bacteriophages Annihilus, TonyStarch, Thiqqums, CricKo, ClubPenguin, RosaAsantewaa, and PherryCruz |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
1.2x10^11 pfu/mL |
Pitt Freezer Box# |
83 |
Pitt Freezer Box Grid# |
H7 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |