| Discovery Information |
| Isolation Host | Streptomyces scabiei RL-34 |
| Found By | Chih Shiang Keith Shee, John Varghese |
| Year Found | 2018 |
| Location Found | Halethorpe, MD USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 39.257417 N, 76.704278 W Map |
| Discovery Notes | Plaques were obtained from an enrichment of the soil sample, and then purified using plaque assay 3 times. |
| Naming Notes | after Terry Crews. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 24, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3283 |
| Genome length (bp) | 43736 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCCCT |
| GC Content | 61.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BI |
| Subcluster | BI2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | from the soil near the roots of a white oak tree. |
| Morphotype | Siphoviridae |
| Number of Genes | 58 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK686070 |
| Refseq Number | None yet |
| Published in Paper | Park et al 2022, Microbiology Resource Announcements: Complete Genome Sequences of Streptomyces Bacteriophages Annihilus, TonyStarch, Thiqqums, CricKo, ClubPenguin, RosaAsantewaa, and PherryCruz |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.2x10^11 pfu/mL |
| Pitt Freezer Box# |
83 |
| Pitt Freezer Box Grid# |
H7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
