| Detailed Information for Phage Phistory |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Former names | EightFour5 |
| Found By | Elliot Wakefield |
| Year Found | 2016 |
| Location Found | Nyack, NY USA |
| Finding Institution | Alliance University (formerly Nyack College) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.0865 N, 73.931 W Map |
| Discovery Notes | Also worked on by Jesse Gregory and Joshua Schmidt. The soil was isolated on the 21st of September, 2016. The soil was collected from the Moseley car park area on the Nyack College campus. The soil had lots of loose debris. The initial work was done by Elliot and then he was joined by Josh and Jesse half way through the semester. |
| Naming Notes | The reason this project has been named," Eight Four 5," is due to the area code of Nyack being 845. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2872 |
| Genome length (bp) | 53085 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CTCGGGGCAT |
| GC Content | 63.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DN |
| Subcluster | DN1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Alliance University (formerly Nyack College) |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are spread across the plate covering much of the area, but so that you can see almost each of the individual plaques, with the exception of some pools of what I counted to be 3-5 in a given place.
The plaques on the plate are transparent and are not colored. |
| Morphotype | Siphoviridae |
| Number of Genes | 106 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH651185 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
71 |
| Pitt Freezer Box Grid# |
G8 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
