| Detailed Information for Phage Phloodle |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Twinkie |
| Found By | Afnan Allahbaksh, Mahati Thiyagarajan |
| Year Found | 2025 |
| Location Found | VA United States |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.70022 N, 77.61611 W Map |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 1195 |
| Genome length (bp) | 41917 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCCATGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | G |
| Subcluster | G1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Virginia Commonwealth University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Morphotype | Siphoviridae |
| Number of Genes | 61 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
3.1*10^11 PFU/ml |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
