| Detailed Information for Phage PhluffyCoco |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Li(Sherry) Xu |
| Year Found | 2022 |
| Location Found | Boston, MA USA |
| Finding Institution | Tufts University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 42.35588 N, 71.06805 W Map |
| Discovery Notes | The sample was discovered at the dog park in Boston Common and it was under a layer of grass. The soil was fine and had rocks and plant matter in it. |
| Naming Notes | The phage was found in a dog park while walking my sister's dog Coco. Coco is a corgi and she is very fluffy. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 11, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 5162 |
| Genome length (bp) | 38568 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | GAGTTGCCGGCA |
| GC Content | 66.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AS |
| Subcluster | AS3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Tufts University |
| Annotation Status | In GenBank |
| Plaque Notes | "PhluffyCoco" produced a range of differently sized plaques from 1mm to 3mm wide. All plaques consisted of a clear center with turbid margins. Most plaques were circular and symmetrical. |
| Number of Genes | 68 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ999096 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2 * 10^10 PFU/mL |
| Pitt Freezer Box# |
203 |
| Pitt Freezer Box Grid# |
G2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
