| Detailed Information for Phage Pioneer3 |
| Discovery Information |
| Isolation Host | Microbacterium paraoxydans NWU1 |
| Found By | Morgan A. Shipley |
| Year Found | 2016 |
| Location Found | Lincoln, NE USA |
| Finding Institution | Nebraska Wesleyan University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.8 N, 96.6 W Map |
| Discovery Notes | The environmental sample was from a tree berm outside of a dormitory at Nebraska Wesleyan University. This sample produced the phage that completed the program. |
| Naming Notes | I named this phage Pioneer3 because it is the name of the dormitory I live in, as well as where my original environmental sample came from to produce my phage, and 3 is my lucky number. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 25, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1255 |
| Genome length (bp) | 62954 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 3191 bp |
| GC Content | 64.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | ED |
| Subcluster | ED1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Nebraska Wesleyan University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are about 2 mm in diameter and clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 114 |
| Number of tRNAs | 4 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH271310 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.3x10^10 |
| Date of SEA Lysate Titering |
Nov 8, 2016 |
| Pitt Freezer Box# |
45 |
| Pitt Freezer Box Grid# |
F9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
