| Detailed Information for Phage Plagueis |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ignacio Ayoroa |
| Year Found | 2012 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.643667 N, 90.2985 W Map |
| Discovery Notes | The phage was found in parkland, near a waterfall and a pond in a wooded area. The area is near to major walking paths. It is also located near the major road that cuts through the park. |
| Naming Notes | The word Plagueis sounds similar to plague and although this may sound unoriginal, it is to describe its living conditions, which are very unclean. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 25, 2018 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 137 |
| Sanger Finishing Reactions | 9 |
| Genome length (bp) | 41722 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCCATGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | G |
| Subcluster | G1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are very small, with circular shapes. The center of the plaques tend to be a bit cloudy; this is seen throughout most if not all of the plaques. Also, the plaques for the most part are spread out from one another though a few do clump together to form larger areas of plaque. |
| Morphotype | Siphoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH450127 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2012WUSLplagueisKH |
| SEA Lysate Titer |
1.66 x 10^10 |
| Date of SEA Lysate Titering |
Oct 9, 2012 |
| Pitt Freezer Box# |
42 |
| Pitt Freezer Box Grid# |
26 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
