| Detailed Information for Phage Poise | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Payge |
| Found By | Catherine Rautio, Julianna DeSantis, Lisa Nguyenho |
| Year Found | 2016 |
| Location Found | Melrose, MA USA |
| Finding Institution | Merrimack College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.451333 N, 71.076333 W Map |
| Discovery Notes | Coming from a beautiful tomato plants soil, our phage was discovered among the damp bottom of the pot, that was watered every day. Seemingly we thought it would be a disaster taking soil that was store bought for the growing of a plant, however we were in for a surprise. It turned out to be a great success, and has given us nothing but hope for the long lasting strong phage! |
| Naming Notes | Our phage seemed like a victory due to having a rare 3 phage DNA and our titer being extremely high, therefore our original name being "Lil Jackpot". With much thought, we decided Paige was much cuter. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 11, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1816 |
| Genome length (bp) | 64824 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | GCGCGCAGCGC |
| GC Content | 63.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | S |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Merrimack College |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques grew very slow and seemed to be turbid when done. As well as being small. |
| Morphotype | Siphoviridae |
| Number of Genes | 116 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewPoise_1 Poise_2 Poise_3 Poise_4 Poise_5 Poise_6 Poise_7 Poise_8 Poise_9 Poise_10 Poise_11 Poise_12 Poise_13 Poise_14 Poise_15 Poise_16 Poise_17 Poise_18 Poise_19 Poise_20 Poise_21 Poise_22 Poise_23 Poise_24 Poise_25 Poise_26 Poise_27 Poise_28 Poise_29 Poise_30 Poise_31 Poise_32 Poise_33 Poise_34 Poise_35 Poise_36 Poise_37 Poise_38 Poise_39 Poise_40 Poise_41 Poise_42 Poise_43 Poise_44 Poise_45 Poise_46 Poise_47 Poise_48 Poise_49 Poise_50 Poise_51 Poise_52 Poise_53 Poise_54 Poise_55 Poise_56 Poise_57 Poise_58 Poise_59 Poise_60 Poise_61 Poise_62 Poise_63 Poise_64 Poise_65 Poise_66 Poise_67 Poise_68 Poise_69 Poise_70 Poise_71 Poise_72 Poise_73 Poise_74 Poise_75 Poise_76 Poise_77 Poise_78 Poise_79 Poise_80 Poise_81 Poise_82 Poise_83 Poise_84 Poise_85 Poise_86 Poise_87 Poise_88 Poise_89 Poise_90 Poise_91 Poise_92 Poise_93 Poise_94 Poise_95 Poise_96 Poise_97 Poise_98 Poise_99 Poise_100 Poise_101 Poise_102 Poise_103 Poise_104 Poise_105 Poise_106 Poise_107 Poise_108 Poise_109 Poise_110 Poise_111 Poise_112 Poise_113 Poise_114 Poise_115 Poise_116 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN329676 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 8.8x10^10 |
| Pitt Freezer Box# | 73 |
| Pitt Freezer Box Grid# | H3 |
| Available Files | |
| GenBank File for Phamerator | Download |