| Detailed Information for Phage Rattrick |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Caleb Pryor |
| Year Found | 2025 |
| Location Found | Midlothian, VA United States |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.46458 N, 77.62675 W Map |
| Naming Notes | The name Rattrick was inspired by the overall shape observed in the TEM image. The phage makes a rat-like silhouette. The name is a play on the name ‘Patrick,’ to match this observation. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 835 |
| Genome length (bp) | 42498 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCCATGGCAT |
| GC Content | 66.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | G |
| Subcluster | G1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Phage released from both potential lysogens in phage release assay. Likely temperate.
Lysogen lawns are much more resistant to infection by HTL compared to M. smegmatis lawn. Lysogens show turbid spot at 10^0, where M. smeg shows plaques to 10^-7. |
| Other Cluster Members |
|
| Annotating Institution | Virginia Commonwealth University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Plaques that are ~3 mm in diameter. THey are clear and round. |
| Morphotype | Siphoviridae |
| Number of Genes | 64 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
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| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
4.5 * 10^10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
