| Detailed Information for Phage Regigigas |
| Discovery Information |
| Isolation Host | Streptomyces scabiei RL-34 |
| Found By | Brianna Holmes Andre Jackson |
| Year Found | 2023 |
| Location Found | Baltimore, MD USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 26°C |
| GPS Coordinates | 39.255555 N, 76.712261 W Map |
| Naming Notes | Regigigas means slow start and at the beginning of isolation and purification we had a slow start due to not knowing our phage required a longer incubation time |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 1, 2024 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 618 |
| Genome length (bp) | 57235 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCCGCCT |
| GC Content | 58.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BI |
| Subcluster | BI4 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | The lysogeny test revealed Regigigas to be a virulent phage. |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | Being Annotated (Expected completion by 12/31/2025) |
| Plaque Notes | turbid, circular plaques appearing after 48 hours at 30 degrees on S.scabiei |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
171 |
| Pitt Freezer Box Grid# |
E3 |
| Available Files |
| Restriction Digest Picture | Download |
| EM Picture | Download |
