| Detailed Information for Phage Ruriko | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Alba L Prince, Brooke A Bubert, Hailee R Perry |
| Year Found | 2025 |
| Location Found | Lindale, TX United States |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.49 N, 95.42 W Map |
| Discovery Notes | The soil sample from which phage Ruriko was isolated was collected at 7:25 am CST on August 26, 2025, from a compost bin in the backyard of a home in rural Lindale, Texas. The morning was partly cloudy with an ambient temperature of 22 °C. Clearing away a layer of decaying fruit and leaves revealed moist soil teeming with worms, bugs, larvae, and the remnants of banana peels, watermelon rinds, corn husks, and coffee grounds. A plastic spoon was used to make a 4-inch wide and 3-inch deep hole, scoop a soil sample into a Ziploc sandwich bag, and take it to the laboratory for processing that morning. Five grams of the soil sample were weighed out and used for further processing, using the enriched isolation method. |
| Naming Notes | Ruriko is a feminine Japanese given name meaning Lapis Lazuli child. Lapis Lazuli is a blue variety of corundum, a type of sapphire, and is Alba's favorite gemstone. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 1036 |
| Genome length (bp) | 41903 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCCATGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | G |
| Subcluster | G1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewJeilious (None) Neoh (None) Angel (G1) Annihilator (G1) Aroostook (G1) Avrafan (G1) AzulaCat (G1) Barkley26 (G1) BPs (G1) BQuat (G1) BruceB (G1) Camri (G1) CassieYates (G1) Cedasite (G1) Chance64 (G1) Cherrybomb426 (G1) CLED96 (G1) Coleslaw (G1) Crespo (G1) Darionha (G1) DingDing (G1) DMoney (G1) ECartman (G1) Frosty24 (G1) Gideon (G1) GoldenAsh (G1) Gomashi (G1) Grizzly (G1) Halo (G1) Hope (G1) Hotshotbaby7 (G1) Jane (G1) Jolene (G1) Jonghyun (G1) JorRay (G1) Kareem (G1) Kasen3 (G1) Liefie (G1) LouisV14 (G1) Maliketh (G1) Marmie (G1) Mowgli (G1) OctaviousRex (G1) Olga (G1) Paito (G1) Peeb (G1) Periodt (G1) Phish (G1) Phloodle (G1) Phreak (G1) PinkYoshi (G1) Plagueis (G1) Rabbs (G1) Rattrick (G1) Remy19 (G1) Renaissance (G1) Richarlison (G1) Ruriko (G1) Schiebel (G1) ShaboiShabazz (G1) Sizemore (G1) Sneeze (G1) Sweets (G1) Taheera (G1) Terror (G1) TinaBug (G1) TomBrady (G1) Wendigo (G1) ZoMa (G1) Zombie (G1) Avocado (G2) Cambiare (G2) FlagStaff (G2) GodSpeed (G2) Pace1224 (G2) Cloudyecho (G3) MOOREtheMARYer (G3) Pinnie (G3) Jolie2 (G4) Lemuria (G4) Mercurio (G4) Antsirabe (G5) Stargaze (G5) YangYin (G5) |
| Annotating Institution | LeTourneau University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Ruriko produced small, bullseye, clear plaques. They were approximately 1.5 mm in diameter (range 0.7-2.1 mm; n = 10). |
| Number of Genes | 60 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewRuriko_1 Ruriko_2 Ruriko_3 Ruriko_4 Ruriko_5 Ruriko_6 Ruriko_7 Ruriko_8 Ruriko_9 Ruriko_10 Ruriko_11 Ruriko_12 Ruriko_13 Ruriko_14 Ruriko_15 Ruriko_16 Ruriko_17 Ruriko_18 Ruriko_19 Ruriko_20 Ruriko_21 Ruriko_22 Ruriko_23 Ruriko_24 Ruriko_25 Ruriko_26 Ruriko_27 Ruriko_28 Ruriko_29 Ruriko_30 Ruriko_31 Ruriko_32 Ruriko_33 Ruriko_34 Ruriko_35 Ruriko_36 Ruriko_37 Ruriko_38 Ruriko_39 Ruriko_40 Ruriko_41 Ruriko_42 Ruriko_43 Ruriko_44 Ruriko_45 Ruriko_46 Ruriko_47 Ruriko_48 Ruriko_49 Ruriko_50 Ruriko_51 Ruriko_52 Ruriko_53 Ruriko_54 Ruriko_55 Ruriko_56 Ruriko_57 Ruriko_58 Ruriko_59 Ruriko_60 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 1x10^10 PFU/ml |