| Detailed Information for Phage Sangak | |
| Discovery Information | |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Found By | Daniel Mirahmadian |
| Year Found | 2024 |
| Location Found | Timonium, MD United States |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.416737 N, 76.617706 W Map |
| Discovery Notes | Flat and rocky terrain. Minimal shade. Dry and dusty soil. |
| Naming Notes | The meaning of "sangak" in Farsi is "pebble". This is in reference to a common flatbread baked in Iran. The dough is thinly laid out on a bed of hot pebbles and is typically eaten fresh. The inspiration for this name came from the rocky nature of the soil samples that the phage was isolated from. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 4, 2025 |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NovaSeq X Plus |
| Read Type | Paired-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 11462 |
| Genome length (bp) | 43248 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCCCT |
| GC Content | 60.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BI |
| Subcluster | BI2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lysogeny testing over a seven day period utilizing spot tests revealed that Sangak is most likely a virulent phage. |
| Other Cluster Members |
Click to ViewCherryBlossom (BI1) DrGrey (BI1) Esketit (BI1) FidgetOrca (BI1) FrodoSwaggins (BI1) GirlPower (BI1) HazuAndZazu (BI1) Hoshi (BI1) IceWarrior (BI1) Indigenous (BI1) Jaylociraptor (BI1) Madamato (BI1) Maya (BI1) Meibysrarus (BI1) Namo (BI1) OlympicHelado (BI1) Popy (BI1) Rima (BI1) Soshi (BI1) Spectropatronm (BI1) TaidaOne (BI1) TieDye (BI1) TonyStarch (BI1) Annihilus (BI2) Bilo (BI2) Doxi13 (BI2) GoblinVoyage (BI2) HotFries (BI2) Moozy (BI2) PherryCruz (BI2) RavenPuff (BI2) RosaAsantewaa (BI2) Sangak (BI2) Scap1 (BI2) SheRa (BI2) LibertyBell (BI3) CricKo (BI4) FromIndy (BI4) Miek (BI4) Rainydai (BI4) Regigigas (BI4) SendItCS (BI4) Thiqqums (BI4) Bing (BI5) Kardashian (BI6) ClubPenguin (BI7) |
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | Being Annotated (Expected completion by 12/4/2025) |
| Plaque Notes | 1-2 mm diameter on average after 48 hours at 30 degrees Celsius on S. mirabilis. Irregular borders and asymmetrical. |
| Morphotype | Siphoviridae |
| Number of Genes | 58 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewSangak_1 Sangak_2 Sangak_3 Sangak_4 Sangak_5 Sangak_6 Sangak_7 Sangak_8 Sangak_9 Sangak_10 Sangak_11 Sangak_12 Sangak_13 Sangak_14 Sangak_15 Sangak_16 Sangak_17 Sangak_18 Sangak_19 Sangak_20 Sangak_21 Sangak_22 Sangak_23 Sangak_24 Sangak_25 Sangak_26 Sangak_27 Sangak_28 Sangak_29 Sangak_30 Sangak_31 Sangak_32 Sangak_33 Sangak_34 Sangak_35 Sangak_36 Sangak_37 Sangak_38 Sangak_39 Sangak_40 Sangak_41 Sangak_42 Sangak_43 Sangak_44 Sangak_45 Sangak_46 Sangak_47 Sangak_48 Sangak_49 Sangak_50 Sangak_51 Sangak_52 Sangak_53 Sangak_54 Sangak_55 Sangak_56 Sangak_57 Sangak_58 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.8 * 10^9 pfu/mL |
| Pitt Freezer Box# | 212 |
| Pitt Freezer Box Grid# | B12 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
