| Detailed Information for Phage Saphira | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Sarah Heady |
| Year Found | 2015 |
| Location Found | Waco, TX USA |
| Finding Institution | Baylor University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 38°C |
| GPS Coordinates | 31.547986 N, 97.120278 W Map |
| Discovery Notes | Dry soil was collected from a location under a tree next to an Exxon gas station near the campus of Baylor University in Waco, TX by Rozi H. The soil was enriched by Mackenzie Kallemeyn in fall 2014 and she archive DaBears. EeyoreJr was also isolated from this soil lysate. |
| Naming Notes | Under Mackenzie Kallemeyn, the phage was named "DaBears," but was never cataloged. Sarah Heady renamed the phage "Saphira" after the dragon in Eragon. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 28, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1348 |
| Genome length (bp) | 15549 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCGCGCCACC |
| GC Content | 60.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AN |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewArby Arielagos Azathoth Blair CGermain Chestnut Copper Courtney3 Decurro Dewayne Elkhorn Guntur Hunnie Inspire2 Jessica Kels KylieMac Laila Link Lore LouisXIV Maggie Mariposa Massimo Moloch Muttlie Prospero Ronnie Sandman Saphira SerialPhiller Seume Sourignavong StewieGriff Stratus Swenson Taj14 TinoCrisci Toulouse TymAbreu Yank |
| Annotating Institution | Neumann University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques produced by Saphira are consistently very small, smooth, and round, with an average diameter of 1-2mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 27 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewSaphira_1 Saphira_2 Saphira_3 Saphira_4 Saphira_5 Saphira_6 Saphira_7 Saphira_8 Saphira_9 Saphira_10 Saphira_11 Saphira_12 Saphira_13 Saphira_14 Saphira_15 Saphira_16 Saphira_17 Saphira_18 Saphira_19 Saphira_20 Saphira_21 Saphira_22 Saphira_23 Saphira_24 Saphira_25 Saphira_26 Saphira_27 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN617842 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.5*10^8 PFU/mL |
| Date of SEA Lysate Titering | Nov 24, 2015 |
| Pitt Freezer Box# | 21 |
| Pitt Freezer Box Grid# | D11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
