| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Montana Heath Simpson |
| Year Found | 2019 |
| Location Found | Clarksville, TN United States |
| Finding Institution | Austin Peay State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 21°C |
| GPS Coordinates | 36.541667 N, 87.367725 W Map |
| Discovery Notes | Time of Day: 10:15 am
Location: Between the Waffle House and Electric Cowboy on Riverside Dr.
Description of Location: Below walking track and extracted from river bank.
Depth: 0.5 to 2.0 cm |
| Naming Notes | I obtained the phage sample from an environmental sample which was extracted from the side of the river bank where the Cumberland River merges with the Red Rivers. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 23, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 73 |
| Genome length (bp) | 53276 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EC |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Austin Peay State University |
| Annotation Status | In GenBank |
| Plaque Notes | Small plaque morphology manly lytic plaques have been observed, but occasional lysogeneic plaques also have been observed. |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT818415 |
| Refseq Number | None yet |
| Published in Paper | Markov et al 2021, Microbiology Resource Announcements: Complete Genome Sequences of Microbacterium Bacteriophages Danno, Otwor, and Scumberland, Isolated in Clarksville, Tennessee |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.0 x 10^9 |
| Pitt Freezer Box# |
97 |
| Pitt Freezer Box Grid# |
H11 |
| Available Files |
| GenBank File for Phamerator | Download |
