| Detailed Information for Phage Soshi |
| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Carrie Yue Shi |
| Year Found | 2018 |
| Location Found | North Richland Hills, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 32.967553 N, 97.267263 W Map |
| Discovery Notes | Soil: very moist, almost like mud, dark brown
Temp: 81 Fahrenheit/ 27.2 Celsius
Area description: Rainy, wet
Time: 9/3/18 @ 1:25-1:30pm |
| Naming Notes | This phage name came from the combination of me and my friend's last name So (friend) Shi (me) |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1818 |
| Genome length (bp) | 56311 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCCGCCT |
| GC Content | 59.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BI |
| Subcluster | BI1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lysogeny was performed. The results seem positive. Will need further tests. |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | Average plaque size: 1 millimeters
Round shape
Slight cloudiness, mainly clear |
| Number of Genes | 85 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN204504 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2x10^10pfu/mL |
| Pitt Freezer Box# |
75 |
| Pitt Freezer Box Grid# |
F5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
