| Detailed Information for Phage TinaBug | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Sophie Norsworthy |
| Year Found | 2024 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.97815 N, 86.41795 W Map |
| Discovery Notes | Soil collected from a potted plant outside of a house. The soil was loose and the plant contained in the soil appeared healthy. The site was free of contaminants. |
| Naming Notes | Named after a combination of my niece's nicknames. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 17, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 5029 |
| Genome length (bp) | 42289 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCCATGGCAT |
| GC Content | 66.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | G |
| Subcluster | G1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAngel (G1) Annihilator (G1) Aroostook (G1) Avrafan (G1) AzulaCat (G1) Barkley26 (G1) BPs (G1) BQuat (G1) BruceB (G1) Camri (G1) CassieYates (G1) Cedasite (G1) Chance64 (G1) Cherrybomb426 (G1) CLED96 (G1) Coleslaw (G1) Crespo (G1) Darionha (G1) DMoney (G1) ECartman (G1) Frosty24 (G1) Gideon (G1) GoldenAsh (G1) Gomashi (G1) Grizzly (G1) Halo (G1) Hope (G1) Hotshotbaby7 (G1) Jane (G1) Jolene (G1) Jonghyun (G1) JorRay (G1) Kareem (G1) Kasen3 (G1) Liefie (G1) LouisV14 (G1) Maliketh (G1) Marmie (G1) Mowgli (G1) OctaviousRex (G1) Olga (G1) Paito (G1) Peeb (G1) Periodt (G1) Phish (G1) Phreak (G1) PinkYoshi (G1) Plagueis (G1) Rabbs (G1) Remy19 (G1) Renaissance (G1) Schiebel (G1) ShaboiShabazz (G1) Sizemore (G1) Sneeze (G1) Sweets (G1) Taheera (G1) Terror (G1) TinaBug (G1) TomBrady (G1) Wendigo (G1) ZoMa (G1) Zombie (G1) Avocado (G2) Cambiare (G2) FlagStaff (G2) Pace1224 (G2) MOOREtheMARYer (G3) Pinnie (G3) Jolie2 (G4) Lemuria (G4) Mercurio (G4) Antsirabe (G5) Stargaze (G5) |
| Annotating Institution | Western Kentucky University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | TinaBug has clear plaques that measure around 3 millimeters in diameter |
| Morphotype | Siphoviridae |
| Number of Genes | 61 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewTinaBug_1 TinaBug_2 TinaBug_3 TinaBug_4 TinaBug_5 TinaBug_6 TinaBug_7 TinaBug_8 TinaBug_9 TinaBug_10 TinaBug_11 TinaBug_12 TinaBug_13 TinaBug_14 TinaBug_15 TinaBug_16 TinaBug_17 TinaBug_18 TinaBug_19 TinaBug_20 TinaBug_21 TinaBug_22 TinaBug_23 TinaBug_24 TinaBug_25 TinaBug_26 TinaBug_27 TinaBug_28 TinaBug_29 TinaBug_30 TinaBug_31 TinaBug_32 TinaBug_33 TinaBug_34 TinaBug_35 TinaBug_36 TinaBug_37 TinaBug_38 TinaBug_39 TinaBug_40 TinaBug_41 TinaBug_42 TinaBug_43 TinaBug_44 TinaBug_45 TinaBug_46 TinaBug_47 TinaBug_48 TinaBug_49 TinaBug_50 TinaBug_51 TinaBug_52 TinaBug_53 TinaBug_54 TinaBug_55 TinaBug_56 TinaBug_57 TinaBug_58 TinaBug_59 TinaBug_60 TinaBug_61 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 7.6 * 10^10 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
