| Discovery Information |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Found By | Heer and Dominique |
| Year Found | 2021 |
| Location Found | Ellicott City, MD United States |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.262 N, 76.818 W Map |
| Discovery Notes | From moist, brown soil from a rose plant pot collected by Heer in her backyard of her house on 9/13/21 at 75 F. |
| Naming Notes | The name was inspired by Marvel character Tony Stark aka Iron Man. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 3, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2535 |
| Genome length (bp) | 55469 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCCGCCT |
| GC Content | 59.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BI |
| Subcluster | BI1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | Clear, round, ~1 mm plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 85 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON108646 |
| Refseq Number | None yet |
| Published in Paper | Park et al 2022, Microbiology Resource Announcements: Complete Genome Sequences of Streptomyces Bacteriophages Annihilus, TonyStarch, Thiqqums, CricKo, ClubPenguin, RosaAsantewaa, and PherryCruz |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.3 * 10^9 |
| Pitt Freezer Box# |
132 |
| Pitt Freezer Box Grid# |
C9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
