| Detailed Information for Phage VasuNzinga |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Vasupradha Girish, Nzinga Walker |
| Year Found | 2014 |
| Location Found | West Lafayette, IN USA |
| Finding Institution | Purdue University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.420934 N, 86.92076 W Map |
| Discovery Notes | Sample collected on 09/01/14, at 1:10 pm. The depth of the soil was around 3.5-4 cm. The approximate moisture of the soil was 70%. Was on the side of a path, covered with leaves. HTL titre: 7.5*10^7 pfu/ml. |
| Naming Notes | Named after the two people who isolated the phage: Vasupradha Girish and Nzinga Walker. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 6, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1246 |
| Genome length (bp) | 64911 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | GCGCGCAGCGC |
| GC Content | 63.4% |
| Sequencing Notes | This phage was identified as being uncommon during a large DOGEMS screening run, then subsequently grown from frozen stock and sequenced.
|
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | S |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Purdue University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque was around 2mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 113 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH727562 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
12 |
| Pitt Freezer Box Grid# |
A12 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
