| Detailed Information for Phage Wigberry |
| Discovery Information |
| Isolation Host | Streptomyces lividans NRRL B-12275 |
| Found By | Allison Wright |
| Year Found | 2025 |
| Location Found | Aubrey, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.351124 N, 97.035042 W Map |
| Discovery Notes | Collected soil from the Ray Roberts Greenbelt Trailhead in Aubrey, Texas on August 19th, 2025. The temperature outside was 101 degrees F with a 35% humidity at 15:30. It was very dry. |
| Naming Notes | I chose the name Wigberry because it is a whimsical name |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 22, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 576 |
| Genome length (bp) | 56900 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCCGCCT |
| GC Content | 59.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BI |
| Subcluster | BI1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Bacteriophage Release yielded positive results
Lysogen Confirmation via Superinfection Immunity yielded results that stated that all candidates were false lysogens. |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | The plaques were mostly clear and about 0.5mm in dimeter |
| Number of Genes | 91 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
1x10^10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
