| Detailed Information for Phage Zhuangyuan |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Yilin Chen |
| Year Found | 2024 |
| Location Found | LOS ANGELES, CA United States |
| Finding Institution | University of Southern California |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.053691 N, 118.242766 W Map |
| Discovery Notes | The phage sample was extracted from the soil on the pathway near the right side of the Leavey Library at the USC Park Campus |
| Naming Notes | The name of my phage comes from my cat, Zhuangyuan, symbolizing the highest honor in the imperial examinations of ancient China. I hope this phage will provide valuable insights as we explore its potential to combat antibiotic-resistant bacteria, contributing to advancements in medicine. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 5980 |
| Genome length (bp) | 40260 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | GAGTTGCCGGCA |
| GC Content | 65.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AS |
| Subcluster | AS2 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Based on the webbed plate that the lysate was collected from, the phage is pretty lytic. |
| Other Cluster Members |
|
| Annotating Institution | University of Southern California |
| Annotation Status | Being Annotated (Expected completion by 12/15/2025) |
| Morphotype | Siphoviridae |
| Number of Genes | 77 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2*10^10 pku/mL |
| Pitt Freezer Box# |
191 |
| Pitt Freezer Box Grid# |
H4 |