Rhodococcus phage AppleCloud
AppleCloud EM Picture
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Detailed Information for Phage AppleCloud
Discovery Information
Isolation HostRhodococcus erythropolis RIA 643
Found ByKristin Graham and McKenzi Lorrig
Year Found2014
Location FoundHudson, WI USA
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature30°C
GPS Coordinates44.984444 N, 92.754444 W Map
Discovery Notesfrom same soil sample as Alatin, flower garden soil, semi-dry and loose
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 7, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage488
Genome length (bp)46389
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGGCCGTGAT
End Determination NotesBuildup of reads in Illumina sequencing data.
GC Content58.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCA
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque Noteslarge, clear plaques, no halo, 2 mm diameter, appearing after 24 hr culture at 30oC
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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AppleCloud_1

AppleCloud_2

AppleCloud_5
(lysin A)

AppleCloud_6
(terminase)

AppleCloud_7
(portal protein)

AppleCloud_8
(capsid maturation protease)

AppleCloud_9
(scaffolding protein)

AppleCloud_10
(major capsid protein)

AppleCloud_11
(head to tail connector protein)

AppleCloud_12
(head to tail connector protein)

AppleCloud_13
(head to tail connector protein)

AppleCloud_14
(head to tail connector protein)

AppleCloud_15
(head to tail connector protein)

AppleCloud_16
(major tail protein)

AppleCloud_17
(tail assembly chaperone)

AppleCloud_18
(tail assembly chaperone)

AppleCloud_19
(tape measure protein)

AppleCloud_20
(minor tail protein)

AppleCloud_21
(minor tail protein)

AppleCloud_22

AppleCloud_23
(holin)

AppleCloud_24

AppleCloud_25

AppleCloud_26

AppleCloud_27
(integrase)

AppleCloud_28
(excise)

AppleCloud_29

AppleCloud_30
(deoxycytidylate deaminase)

AppleCloud_31

AppleCloud_32

AppleCloud_33

AppleCloud_34
(DNA polymerase I)

AppleCloud_35

AppleCloud_36
(HTH DNA binding domain protein)

AppleCloud_37
(thymidylate synthase)

AppleCloud_38
(ribonucleotide reductase)

AppleCloud_39

AppleCloud_40
(DNA binding protein)

AppleCloud_41
(metallophosphoesterase)

AppleCloud_42

AppleCloud_43
(glutaredoxin)

AppleCloud_44

AppleCloud_45
(DNA primase)

AppleCloud_46
(DNA primase)

AppleCloud_47

AppleCloud_48
(endonuclease VII)

AppleCloud_49

AppleCloud_50
(DnaB-like helicase)

AppleCloud_51

AppleCloud_52

AppleCloud_53
(RecB-like exonuclease)

AppleCloud_54

AppleCloud_55
(HTH DNA binding protein)

AppleCloud_56

AppleCloud_57

AppleCloud_58

AppleCloud_59

AppleCloud_60

AppleCloud_61
(ArdA-like antirestriction protein)

AppleCloud_62

AppleCloud_63

AppleCloud_64

AppleCloud_65

AppleCloud_66

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF324903
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.2 x 10^9
Date of SEA Lysate Titering Oct 20, 2014
Pitt Freezer Box# 12
Pitt Freezer Box Grid# G9
Available Files
GenBank File for PhameratorDownload