| Detailed Information for Phage Rhodalysa |
| Discovery Information |
| Isolation Host | Rhodococcus erythropolis RIA 643 |
| Found By | Juliet Downie |
| Year Found | 2014 |
| Location Found | Williamsburg, VA USA |
| Finding Institution | College of William & Mary |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.282925 N, 76.664688 W Map |
| Discovery Notes | Soil sample was isolated from compost soil. It underwent two rounds of purification before beginning web lysis process.
Phage measurements:
Capsid diameter = 60-70 nm
Tail length = 150-200 nm |
| Naming Notes | The bacterial host was Rhodococcus globerulus; the phage lyses the bacteria, so it is a "lyser" or "lysa" of "Rhoda", hence "Rhoda""lyser", or "Rhodalysa". I also liked how this name can be pronounced "rhoda-LEE-sah", so it rhymes with Mona Lisa. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 28, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2782 |
| Genome length (bp) | 46596 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGTGAT |
| GC Content | 58.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CA |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques appeared small, clear, and circular. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT375356 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
6.10 x 10^8 PFU/mL |
| Date of SEA Lysate Titering |
Nov 4, 2014 |
| Pitt Freezer Box# |
9 |
| Pitt Freezer Box Grid# |
G12 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
