Rhodococcus phage Rhodalysa
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Detailed Information for Phage Rhodalysa
Discovery Information
Isolation HostRhodococcus erythropolis RIA 643
Found ByJuliet Downie
Year Found2014
Location FoundWilliamsburg, VA United States
Finding InstitutionCollege of William & Mary
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates37.282925 N, 76.664688 W Map
Discovery NotesSoil sample was isolated from compost soil. It underwent two rounds of purification before beginning web lysis process.

Phage measurements:
Capsid diameter = 60-70 nm
Tail length = 150-200 nm
Naming NotesThe bacterial host was Rhodococcus globerulus; the phage lyses the bacteria, so it is a "lyser" or "lysa" of "Rhoda", hence "Rhoda""lyser", or "Rhodalysa". I also liked how this name can be pronounced "rhoda-LEE-sah", so it rhymes with Mona Lisa.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 28, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2782
Genome length (bp)46596
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCCGTGAT
GC Content58.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCA
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesPlaques appeared small, clear, and circular.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT375356
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 6.10 x 10^8 PFU/mL
Date of SEA Lysate Titering Nov 4, 2014
Pitt Freezer Box# 9
Pitt Freezer Box Grid# G12
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload