| Detailed Information for Phage BeautPeep30 |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Former names | PhruitLoop |
| Found By | Sydni Ernst, Joseph Rajasekaran |
| Year Found | 2019 |
| Location Found | Philadelphia, PA United States of America |
| Finding Institution | Drexel University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 28°C |
| GPS Coordinates | 39.953 N, 75.186 W Map |
| Discovery Notes | Dry soil collected from under mulch, a few feet away from a short, young tree. Many weak roots were avoided when collecting the sample. General Vicinity: under blue Drexel University Bridge. |
| Naming Notes | The phage PhruitLoop came from the cat Fruit Lööp. Since umlauts and other special characters cannot be used, the name PhruitLoop was used instead. The "Ph" is a pun on the word "phage."
The name PhruitLoop was vetoed as it was a homophone of the phage named FruitLoop.
The name BeautPeep30 originates from the song "Beautiful People" by Ed Sheeran. The number 30 was chosen because it was found on 30th street in Philadelphia. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 1, 2019 |
| Approximate Shotgun Coverage | 1823 |
| Genome length (bp) | 41809 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Drexel University |
| Annotation Status | In GenBank |
| Plaque Notes | Initially, the first positive direct isolation of the phage with the M.foliorum host produced a singular clear plaque with sharp edges. Upon purification by serial dilution, more plaques formed with two distinct diameters but otherwise identical morphologies. The larger of the two plaques was ~1.0 cm in diameter and the smaller was ~0.5 cm in diameter. The smaller of the plaques was picked and further purified to produce a pure phage population with consistent diameters of ~0.5cm. |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT310861 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
8.4 x 10^9 pfu/mL |
| Pitt Freezer Box# |
105 |
| Pitt Freezer Box Grid# |
F8 |
| Available Files |
| GenBank File for Phamerator | Download |