Gordonia phage Zarbodnamra
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Detailed Information for Phage Zarbodnamra
Discovery Information
Isolation HostGordonia terrae 3612
Found ByArmando Braz
Year Found2017
Location FoundBethlehem, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates40.65659 N, 75.342957 W Map
Discovery NotesLocation: This soil sample was collected from my hometown of Bethlehem, PA just prior to the start of the semester. I wanted to see if I'd see any luck with some soil from over 300 miles away, just out of curiosity.

The Soil Conditions: I retrieved this soil from my vegetable garden that had been used the previous summer. This soil is used annually to grow tomatoes, peppers, cucumbers, parsley, broccoli and other herbs/vegetables. Every year I use miracle grow nutrients in order to give the plants more of what they need to grow. On the day of soil collection (Jan 8, 2017), the sol was very dry and frozen. The soil appeared "deadened" in that the soil was a gray brown colour and was not very cohesive (sandy/loamy texture). There was a lot of plant detritus mixed in with the soil.

Weather Conditions: At the time of collection, air temperature was -6 degrees celcius. 3 days prior there was a snow storm, with snow from that still present on the ground. Weather over the past 24 hours involved blustering winds, with freezing rain overnight. During the actual collection of the soil however, skies were clear.
Naming NoteszarBodnamra comes from a nickname my South African friend would always call me in primary school. It has a catchy ring to it and I like it. Note that the "r's" are silent.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 16, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1504
Genome length (bp)49729
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGCCGGTGA
GC Content67.3%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusOverdue (Expected completion by 5/15/2018)
Plaque NotesMy phage produced clear plaques that vary in diameter size, ranging from diameter = .5mm to diameter = 3mm. The plaques are for the most part entirely circular and form a well defined boundary between where cells underwent lysis, and where cells did not.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 56
Pitt Freezer Box Grid# F10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload