| Detailed Information for Phage Blino | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Abigail Dean |
| Year Found | 2019 |
| Location Found | Pittsburgh, PA United States |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.4464 N, 79.9553 W Map |
| Discovery Notes | Location - Oakland, Pennsylvania (40.4464, -79.9553) Date - 2019.9.17 Time - (3:30 PM) Weather Conditions - Partly Cloudy, Humidity 75%, Pressure 30.01 inHg, Recently rained, Air Quality Good, UV Index 5 Temperature - 78 degrees F Surrounding Area - Soil under the mulch behind the Wyndham Hotel. It was a more moist patch of soil and found at the base of some landscaped bushes and grass. Transport Conditions Duration - 0.5 hours Temperature - 70 degrees inside a backpack Exposure to Sunlight - none |
| Naming Notes | Blino means tired or irritating in Welsh, which is the experience I had in finding and purifying this phage. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 20, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1211 |
| Genome length (bp) | 54168 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TGGCCGGTGA |
| GC Content | 66.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CV |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewArchis Azula Barco Begonia BlingBling Blino Blueberry CaptainKirk2 CarolAnn Delian Fairfaxidum Fenry Frokostdame Gambino Guacamole Hitter Jalammah JasperJr JuJu KappaFarmDelta Lysidious Malachai Melba Mellie MintFen MissRona Murp Obliviate PCoral7 Petra PhrostedPhlake Pipp PrincePatrick Samba Toast UmaThurman Utz Walrus William Wisp Wocket Zarbodnamra ZiggyZoo |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Fully grown plaques are around 1.8mm in diameter. They are clear plaques, not cloudy or having a halo, with relatively definite circular edges. The shape is not entirely perfectly circular. |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewBlino_1 Blino_2 Blino_3 Blino_4 Blino_5 Blino_6 Blino_7 Blino_8 Blino_9 Blino_10 Blino_11 Blino_12 Blino_13 Blino_14 Blino_15 Blino_16 Blino_17 Blino_18 Blino_19 Blino_20 Blino_21 Blino_22 Blino_23 Blino_24 Blino_25 Blino_26 Blino_27 Blino_28 Blino_29 Blino_30 Blino_31 Blino_32 Blino_33 Blino_34 Blino_35 Blino_36 Blino_37 Blino_38 Blino_39 Blino_40 Blino_41 Blino_42 Blino_43 Blino_44 Blino_45 Blino_46 Blino_47 Blino_48 Blino_49 Blino_50 Blino_51 Blino_52 Blino_53 Blino_54 Blino_55 Blino_56 Blino_57 Blino_58 Blino_59 Blino_60 Blino_61 Blino_62 Blino_63 Blino_64 Blino_65 Blino_66 Blino_67 Blino_68 Blino_69 Blino_70 Blino_71 Blino_72 Blino_73 Blino_74 Blino_75 Blino_76 Blino_77 Blino_78 Blino_79 Blino_80 Blino_81 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW291016 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.6x10^9 |
| Pitt Freezer Box# | 113 |
| Pitt Freezer Box Grid# | G9 |
| Available Files | |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |