| Detailed Information for Phage Akoni | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Alexis Bailey |
| Year Found | 2017 |
| Location Found | Tampa, FL USA |
| Finding Institution | University of South Florida |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 28.124411 N, 82.368309 W Map |
| Discovery Notes | The conditions where and when I got my soil were somewhat ideal for soil collection. The soil was next to lake and dock at a natural park. Soil was collected from underneath a dead snail shell. The weather was typically Floridian weather in which it had just rained and was damp outside. |
| Naming Notes | I wanted my phage to have a name with meaning behind it. At first it was going to be the word "difficult" in Latin because of how stubborn it was to make plaques. However, I came across the word Akoni which means "worthy of admiration or praise" which I believe this phage does because of its persistence. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 28, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 261 |
| Genome length (bp) | 54307 |
| Character of genome ends | Circularly Permuted |
| GC Content | 60.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EK |
| Subcluster | EK2 |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
Click to ViewEugeneKrabs (None) KingKamren (None) Zhengyi (None) Alex44 (EK1) ArMaWen (EK1) Biozilla (EK1) Birdfeeder (EK1) BlueRugrat (EK1) Corn21 (EK1) CrunchyBoi (EK1) Dashyla (EK1) DumpQuist (EK1) HitchHiker (EK1) LesNorah (EK1) LilyLou (EK1) Oatly (EK1) Pabst (EK1) Phogo (EK1) PineapplePluto (EK1) Stormbreaker (EK1) TinyTimothy (EK1) Unphazed (EK1) Wesak (EK1) Xitlalli (EK1) YellowPanda (EK1) Akoni (EK2) AloeVera (EK2) Ashton (EK2) Astartes (EK2) Atraxi (EK2) Barroma (EK2) Fede (EK2) Fullmetal (EK2) JordanFarm (EK2) Mazun (EK2) Moleficent (EK2) Morrill (EK2) Pharky (EK2) Phedro (EK2) Phractured (EK2) PhriedRice (EK2) RicoCaldo (EK2) StagePhright (EK2) ThirteenKH (EK2) TrippleS (EK2) Truong (EK2) Waterlily (EK2) Yafa (EK2) |
| Annotating Institution | University of South Florida |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques that grew in the beginning were not as clear; however, after the purification rounds it became constant where the plaques were extremely tiny and clear making them lytic. |
| Morphotype | Podoviridae |
| Number of Genes | 55 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewAkoni_1 Akoni_2 Akoni_3 Akoni_4 Akoni_5 Akoni_6 Akoni_7 Akoni_8 Akoni_9 Akoni_10 Akoni_11 Akoni_12 Akoni_13 Akoni_14 Akoni_15 Akoni_16 Akoni_17 Akoni_18 Akoni_19 Akoni_20 Akoni_21 Akoni_22 Akoni_23 Akoni_24 Akoni_25 Akoni_26 Akoni_27 Akoni_28 Akoni_29 Akoni_30 Akoni_31 Akoni_32 Akoni_33 Akoni_34 Akoni_35 Akoni_36 Akoni_37 Akoni_38 Akoni_39 Akoni_40 Akoni_41 Akoni_42 Akoni_43 Akoni_44 Akoni_45 Akoni_46 Akoni_47 Akoni_48 Akoni_49 Akoni_50 Akoni_51 Akoni_52 Akoni_53 Akoni_54 Akoni_55 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK757449 |
| Refseq Number | None yet |
| Published in Paper | Fakhre et al 2021, Microbiology Resource Announcements: Genome Sequences of Akoni, Ashton, and Truong, Podoviridae Bacteriophages Isolated from Microbacterium foliorum |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 48 |
| Pitt Freezer Box Grid# | G2 |
| Available Files | |
| EM Picture | Download |
| GenBank File for Phamerator | Download |