Microbacterium phage Ashton
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Detailed Information for Phage Ashton
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Former namesZander
Found ByHannah Grzech
Year Found2017
Location FoundTampa, FL USA
Finding InstitutionUniversity of South Florida
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature34°C
GPS Coordinates28.06 N, 82.41967 W Map
Discovery NotesThe soil sample was collected from an area that had been under construction and they had just flattened the up-rooted ground. Also, it was right next to a water drain, so it was black, moist soil with a grey layer underneath. This phage was found through direct isolation.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 21, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage46
Genome length (bp)54519
Character of genome endsCircularly Permuted
GC Content60.1%
Fasta file available?Yes: Download fasta file
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionUniversity of South Florida
Annotation StatusIn GenBank
Plaque NotesPin-sized often turbid plaques that grow very slowly. They are not perfectly round, and are difficult to count on a plaque assay.
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT310875
Refseq NumberNone yet
Published in PaperFakhre et al 2021, Microbiology Resource Announcements: Genome Sequences of Akoni, Ashton, and Truong, Podoviridae Bacteriophages Isolated from Microbacterium foliorum
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.18x10^10 pfu/ml
Pitt Freezer Box# 48
Pitt Freezer Box Grid# G3
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload