Microbacterium phage Pabst
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Detailed Information for Phage Pabst
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByHunter Chase Bishop
Year Found2019
Location FoundCullowhee, NC United States of America
Finding InstitutionWestern Carolina University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates35.309196 N, 83.178029 W Map
Discovery NotesThis phage was discovered in a creek that runs between and under Albright and Benton Residence halls at Western Carolina University. The sample was collected in a shallow silty region of the creek.
Naming NotesThe phage is named after the American Lager beer Pabst Blue Ribbon. Moreover, the name Pabst is a nickname for my father and what he wants to be called as a grandpa.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 2, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage122
Genome length (bp)53620
Character of genome endsCircularly Permuted
GC Content59.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEK
SubclusterEK1
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionWestern Carolina University
Annotation StatusIn GenBank
Plaque NotesMy plaques were very small and would often cluster together giving the appearance of larger plaques than the individual plaques. They averaged 1 mm in diameter. The plaques were best described as "freckle-like".
MorphotypePodoviridae
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT498064
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 2x10^8 PFU/mL
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload