Microbacterium phage CrunchyBoi
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Detailed Information for Phage CrunchyBoi
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByCameron Nicklos and Joshua DeKlotz
Year Found2018
Location FoundSpokane, WA USA
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature25°C
GPS Coordinates47.667222 N, 117.4025 W Map
Discovery NotesPhage found in puddle outside of Hughes Hall at Gonzaga University. Phage enriched and isolated, and grown at room temperature of 25 Degrees Celsius.
Naming NotesSince the phage likes to "eat" bacteria, it was imagined that a "crunchy" sound is made when the bacteria were lysed. Hence the name "CrunchyBoi"
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 7, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage270
Genome length (bp)53800
Character of genome endsCircularly Permuted
GC Content59.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEK
SubclusterEK1
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionGonzaga University
Annotation StatusBeing Annotated (Expected completion by 12/18/2020)
Plaque NotesPlaques were consistently clear and circular. Diameter of plaques ranged from 0.2mm-1.0mm.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 94
Pitt Freezer Box Grid# B10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload