| Detailed Information for Phage YellowPanda | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Travis Kryszewski |
| Year Found | 2021 |
| Location Found | Tampa, FL USA |
| Finding Institution | Hillsborough Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 95°C |
| GPS Coordinates | 28.059883 N, 82.515914 W Map |
| Discovery Notes | Overview- Acquired soil from grass patch within 6 feet of a palm tree that was composted and fully decomposed into the soil a month prior. used sterilized plastic spoon to gather soil, soil was scooped into a ziplock sandwich bag, and lightly mixed to ensure a broad collect from full soil level extracted. * Depth 2 1/2 all soil was used * Water content moist * Light level- very shady, moody light * Temp 95F atmospheric * Rainfall Heavy within the past 6 hours rained * Health - Soil was very healthy, rich, black, teaming with nearby lush greenery and foliage, many insects and a teeming ecosystem. Protocol used 5.2 NOTE- Small changes to protocol, in that some steps were altered or followed differently, the scooping device was a sterilized in isopropyl rubbing alcohol, and ziplock baggy was not inverted and instead had sample directly scooped into it due to it being too small to invert and grasp the soil. Once soil was within baggy it was lightly mixed to ensure bacteria at surface and bottom level of the 2 1/2 inch soil extract were mixed to ensure all bacteria were included. ADDITIONAL NOTE Extraction site had a malodor distinct to Microbacterium foliorum NRRL B-24224 |
| Naming Notes | Yellow panda, it was named "Yellow" given the fact that the bacteria we used to culture it is yellow, Panda due to phage and panda similarities and marketability. A panda only eats bamboo, and only certain species of bamboo, both are highly specialized beings that only eat certain things, and it is also an incredibly friendly name overall. If you say you are to be injecting someone with PRAXUS II they are not going to be as keenly open to the idea as opposed to saying hey were going to cure you by injecting you with yellow Panda. Its very friendly, and I find it builds both on my phages discovery and my fascinating correlation between phage and pandas. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 3, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2135 |
| Genome length (bp) | 53658 |
| Character of genome ends | Circularly Permuted |
| GC Content | 56.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EK |
| Subcluster | EK1 |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
Click to ViewEugeneKrabs (None) Zhengyi (None) Alex44 (EK1) ArMaWen (EK1) Birdfeeder (EK1) BlueRugrat (EK1) Corn21 (EK1) CrunchyBoi (EK1) DumpQuist (EK1) LesNorah (EK1) LilyLou (EK1) Pabst (EK1) Phogo (EK1) PineapplePluto (EK1) Stormbreaker (EK1) TinyTimothy (EK1) Unphazed (EK1) Wesak (EK1) Xitlalli (EK1) YellowPanda (EK1) Akoni (EK2) AloeVera (EK2) Ashton (EK2) Atraxi (EK2) Fede (EK2) Fullmetal (EK2) JordanFarm (EK2) Mazun (EK2) Moleficent (EK2) Morrill (EK2) Pharky (EK2) Phedro (EK2) Phractured (EK2) PhriedRice (EK2) StagePhright (EK2) ThirteenKH (EK2) Truong (EK2) Yafa (EK2) |
| Annotating Institution | Hillsborough Community College |
| Annotation Status | Overdue (Expected completion by 12/1/2022) |
| Plaque Notes | Lytic plaques diameter very small 0.5 mm in diameter to 0.75 mm very voracious usually results in total blowouts if not diluted. |
| Morphotype | Podoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewYellowPanda_Draft_1 YellowPanda_Draft_2 YellowPanda_Draft_3 YellowPanda_Draft_4 YellowPanda_Draft_5 YellowPanda_Draft_6 YellowPanda_Draft_7 YellowPanda_Draft_8 YellowPanda_Draft_9 YellowPanda_Draft_10 YellowPanda_Draft_11 YellowPanda_Draft_12 YellowPanda_Draft_13 YellowPanda_Draft_14 YellowPanda_Draft_15 YellowPanda_Draft_16 YellowPanda_Draft_17 YellowPanda_Draft_18 YellowPanda_Draft_19 YellowPanda_Draft_20 YellowPanda_Draft_21 YellowPanda_Draft_22 YellowPanda_Draft_23 YellowPanda_Draft_24 YellowPanda_Draft_25 YellowPanda_Draft_26 YellowPanda_Draft_27 YellowPanda_Draft_28 YellowPanda_Draft_29 YellowPanda_Draft_30 YellowPanda_Draft_31 YellowPanda_Draft_32 YellowPanda_Draft_33 YellowPanda_Draft_34 YellowPanda_Draft_35 YellowPanda_Draft_36 YellowPanda_Draft_37 YellowPanda_Draft_38 YellowPanda_Draft_39 YellowPanda_Draft_40 YellowPanda_Draft_41 YellowPanda_Draft_42 YellowPanda_Draft_43 YellowPanda_Draft_44 YellowPanda_Draft_45 YellowPanda_Draft_46 YellowPanda_Draft_47 YellowPanda_Draft_48 YellowPanda_Draft_49 YellowPanda_Draft_50 YellowPanda_Draft_51 YellowPanda_Draft_52 YellowPanda_Draft_53 YellowPanda_Draft_54 YellowPanda_Draft_55 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 136 |
| Pitt Freezer Box Grid# | H1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
