Microbacterium phage Akoni
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Detailed Information for Phage Akoni
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByAlexis Bailey
Year Found2017
Location FoundTampa, FL USA
Finding InstitutionUniversity of South Florida
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates28.124411 N, 82.368309 W Map
Discovery NotesThe conditions where and when I got my soil were somewhat ideal for soil collection. The soil was next to lake and dock at a natural park. Soil was collected from underneath a dead snail shell. The weather was typically Floridian weather in which it had just rained and was damp outside.
Naming NotesI wanted my phage to have a name with meaning behind it. At first it was going to be the word "difficult" in Latin because of how stubborn it was to make plaques. However, I came across the word Akoni which means "worthy of admiration or praise" which I believe this phage does because of its persistence.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 28, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage261
Genome length (bp)54307
Character of genome endsCircularly Permuted
GC Content60.1%
Fasta file available?Yes: Download fasta file
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionUniversity of South Florida
Annotation StatusIn GenBank
Plaque NotesThe plaques that grew in the beginning were not as clear; however, after the purification rounds it became constant where the plaques were extremely tiny and clear making them lytic.
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK757449
Refseq NumberNone yet
Published in PaperFakhre et al 2021, Microbiology Resource Announcements: Genome Sequences of Akoni, Ashton, and Truong, Podoviridae Bacteriophages Isolated from Microbacterium foliorum
Archiving Info
Archiving status Archived
Pitt Freezer Box# 48
Pitt Freezer Box Grid# G2
Available Files
EM PictureDownload
GenBank File for PhameratorDownload