| Detailed Information for Phage Aroostook |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Lauren Rockwell |
| Year Found | 2015 |
| Location Found | Longview , TX USA |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.467045 N, 94.724361 W Map |
| Discovery Notes | I collected my environmental sample on campus at LeTourneau University on 9/17/15 at about 8:20 AM. The specific location of where I retrieved my sample was at the base of a bush beside a parking lot in front of a residence hall. The weather was 73°F and sunny. The soil was slightly moist, partly because it was at the base of the plant and also because the ground had dew on it. |
| Naming Notes | Until I moved to Texas four years ago, I had been living in Maine my entire life. I still consider myself a "Mainer" rather than a "Texan," and I always will. Because this place is very special and unique to me, I wanted to name my phage after it. Originally, I wanted to name my phage "Island Falls," the town in which I grew up. However, to give my phage a simpler, one-word name, I chose to name it Aroostook, the county in which Island Falls is located. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 4, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 4385 |
| Genome length (bp) | 41903 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCCATGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | G |
| Subcluster | G1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF668268 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2*10^10 pfu/mL |
| Date of SEA Lysate Titering |
Oct 27, 2015 |
| Pitt Freezer Box# |
21 |
| Pitt Freezer Box Grid# |
E5 |
| Available Files |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
