| Detailed Information for Phage Ashton |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Former names | Zander |
| Found By | Hannah Grzech |
| Year Found | 2017 |
| Location Found | Tampa, FL USA |
| Finding Institution | University of South Florida |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 34°C |
| GPS Coordinates | 28.06 N, 82.41967 W Map |
| Discovery Notes | The soil sample was collected from an area that had been under construction and they had just flattened the up-rooted ground. Also, it was right next to a water drain, so it was black, moist soil with a grey layer underneath. This phage was found through direct isolation. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 21, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 46 |
| Genome length (bp) | 54519 |
| Character of genome ends | Circularly Permuted |
| GC Content | 60.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EK |
| Subcluster | EK2 |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | University of South Florida |
| Annotation Status | In GenBank |
| Plaque Notes | Pin-sized often turbid plaques that grow very slowly. They are not perfectly round, and are difficult to count on a plaque assay. |
| Morphotype | Podoviridae |
| Number of Genes | 56 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT310875 |
| Refseq Number | None yet |
| Published in Paper | Fakhre et al 2021, Microbiology Resource Announcements: Genome Sequences of Akoni, Ashton, and Truong, Podoviridae Bacteriophages Isolated from Microbacterium foliorum |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.18x10^10 pfu/ml |
| Pitt Freezer Box# |
48 |
| Pitt Freezer Box Grid# |
G3 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
