| Detailed Information for Phage Auspice |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Daniel DeBattista, Astrid Etemadi |
| Year Found | 2022 |
| Location Found | Boulder, CO United States |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.009774 N, 105.268996 W Map |
| Discovery Notes | The soil sample was collected from mud at the base of a tree outside the CU Boulder Rec Center. |
| Naming Notes | After a series of problems isolating a phage, finally succeeding felt particularly auspicious. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 13, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 923 |
| Genome length (bp) | 81421 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACCTCCTGCAA |
| GC Content | 61.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | M |
| Subcluster | M1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Colorado Boulder |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were transparent with clearly defined edges. ~1mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 140 |
| Number of tRNAs | 19 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR253916 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
8.7E10 pfu/mL |
| Pitt Freezer Box# |
156 |
| Pitt Freezer Box Grid# |
A10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
