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Mycobacterium phage PegLeg
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Detailed Information for Phage PegLeg
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesMyPhage
Found ByTracy Le et al.
Year Found2011
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramUnavailable
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates34.070578 N, 118.453152 W Map
Discovery NotesIsolated in fall 2011 by Team T-9 and Counting (MIMG 103AL): Tracy Le, Jan Michael Taguiam, Jonathan Tran, and Junhao (Jay) Zhang
Instructor (AL): Todd Lorenz
TA (AL): Jovo Vijanderan
Annotated in fall 2012 by Team Pirates of the CURE-ibbeans (MIMG 103BL): Tiffany Chow, Josephine Nguyen, Sean Chang, and Khristina Ipapo
Instructor (BL): Erin Sanders
TA (BL): Will Villella
Curation in winter 2013 by (MIMG 199): Tiffany Chow, Khristina Ipapo, and Shannon Cooper
Naming NotesThe new phage name was given by Tiffany Chow, Khristina Ipapo, and Shannon Cooper who curated the genome annotation results during winter quarter 2013.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 14, 2013
Sequencing FacilityThe UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage150
Genome length (bp)80955
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceACCTCCTGCAA
GC Content61.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterM
SubclusterM1
Cluster Life CycleLytic
Lysogeny NotesPlaque morphology indicates a lytic life cycle. However, the presence of an integrase gene is an area of interest. Further investigation using bioinformatics indicates that this is a remnant integrase, indicating that it could have recently evolved from a temperate phage.
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesPlaques are round and clear with an average diameter of 2mm. The size range is 1mm to 3mm.
MorphotypeSiphoviridae
Number of Genes139
Number of tRNAs16
Has been Phamerated?Yes
Gene List

Click to View

PegLeg_1

PegLeg_2

PegLeg_3
(Lsr2)

PegLeg_4

PegLeg_5

PegLeg_6

PegLeg_7

PegLeg_8

PegLeg_9

PegLeg_10

PegLeg_11

PegLeg_12

PegLeg_13

PegLeg_14
(terminase)

PegLeg_15
(portal protein)

PegLeg_16
(capsid maturation protease)

PegLeg_17
(scaffolding protein)

PegLeg_18
(capsid protein)

PegLeg_19
(head-to-tail adaptor)

PegLeg_20
(head-to-tail stopper)

PegLeg_21

PegLeg_22
(tail terminator)

PegLeg_23
(major tail subunit)

PegLeg_24
(tail assembly chaperone)

PegLeg_25
(tail assembly chaperone)

PegLeg_26
(tapemeasure protein)

PegLeg_27
(minor tail protein)

PegLeg_28
(minor tail protein)

PegLeg_29
(minor tail protein)

PegLeg_30
(minor tail protein)

PegLeg_31

PegLeg_32

PegLeg_33

PegLeg_34

PegLeg_35
(LysA)

PegLeg_36

PegLeg_37
(holin)

PegLeg_38
(LysB)

PegLeg_39
(NrdH)

PegLeg_40

PegLeg_41

PegLeg_42

PegLeg_43

PegLeg_44
(glutaredoxin)

PegLeg_45

PegLeg_46

PegLeg_47
(NrdC)

PegLeg_48

PegLeg_49

PegLeg_50

PegLeg_51

PegLeg_52

PegLeg_53

PegLeg_54
(ERF protein)

PegLeg_55

PegLeg_56

PegLeg_57

PegLeg_58
(ClpP-like protein)

PegLeg_59

PegLeg_60

PegLeg_61

PegLeg_62

PegLeg_63

PegLeg_64

PegLeg_65

PegLeg_66
(ssDNA binding protein)

PegLeg_67

PegLeg_68
(DnaB-like helicase)

PegLeg_69

PegLeg_70

PegLeg_71
(WhiB)

PegLeg_72

PegLeg_73

PegLeg_74

PegLeg_75

PegLeg_76

PegLeg_77

PegLeg_78
(DnaQ-like protein)

PegLeg_79

PegLeg_80

PegLeg_81

PegLeg_82
(PTPc)

PegLeg_83

PegLeg_84

PegLeg_85

PegLeg_86

PegLeg_87

PegLeg_88
(HNH domain protein)

PegLeg_89

PegLeg_94

PegLeg_96
(HNH domain protein)

PegLeg_102
(HNH endonuclease)

PegLeg_104

PegLeg_107

PegLeg_109

PegLeg_110
(NrdG)

PegLeg_117
(HNH domain protein)

PegLeg_119
(RtcB)

PegLeg_120
(band 7 protein)

PegLeg_121
(MazG)

PegLeg_122

PegLeg_123

PegLeg_124

PegLeg_125

PegLeg_126

PegLeg_127

PegLeg_128

PegLeg_129
(phosphoesterase)

PegLeg_130

PegLeg_131

PegLeg_132

PegLeg_133

PegLeg_134
(integrase)

PegLeg_135

PegLeg_136

PegLeg_137

PegLeg_138

PegLeg_139

PegLeg_140

PegLeg_141

PegLeg_142

PegLeg_143

PegLeg_144

PegLeg_145

PegLeg_146

PegLeg_147

PegLeg_148

PegLeg_149

PegLeg_150

PegLeg_151

PegLeg_152

PegLeg_153

PegLeg_154

PegLeg_155

PegLeg_156
(HNH domain protein)

PegLeg_157

PegLeg_158

PegLeg_159

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKC900379
Refseq NumberNC_021299
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2011UCLAmspb01JT
SEA Lysate Titer 6.4x10^9
Date of SEA Lysate Titering Oct 27, 2011
Pitt Freezer Box# 3
Pitt Freezer Box Grid# E9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload