Mycobacterium phage Skinny
Skinny EM Picture
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Detailed Information for Phage Skinny
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJenna Curran, Kristen Rose
Year Found2021
Location FoundLongview, TX United States
Finding InstitutionLeTourneau University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates32.467601 N, 94.723749 W Map
Discovery NotesWe collected a sample of soil right outside of Speer Chapel on the campus of LeTourneau University. It was cloudy and humid when the sample was collected. It had been raining the night before, so the soil was fairly moist. There were lots of thin roots in the soil, and ants were seen in the surrounding soil. There were also small holes, possibly for snakes or worms, in the surrounding soil.
Naming NotesOne day in lab, we were doing serial dilutions for a round of purification. Dr. Baliraine asked us how we told our smeg cell tubes and our phage buffer tubes apart. We said the phage buffer tubes (with our plaque) had the "skinny" sharpie marker on them. And from there we've called our phage "Skinny."
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 1, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage499
Genome length (bp)82071
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceACCTCCTGCAA
GC Content61.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterM
SubclusterM1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionLeTourneau University
Annotation StatusBeing Annotated (Expected completion by 7/31/2022)
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 134
Pitt Freezer Box Grid# D9