Mycobacterium phage Aziz
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Detailed Information for Phage Aziz
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTrevor Ruesch and Carol Stepniewski
Year Found2017
Location FoundSpokane, WA USA
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature22°C
GPS Coordinates47.667416 N, 117.401525 W Map
Discovery NotesThe isolation was enriched and was conducted at a room temperature of 22 degree C.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 7, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage749
Genome length (bp)83412
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceACCCCATGCAA
GC Content60.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterM
SubclusterM2
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionGonzaga University
Annotation StatusIn GenBank
Plaque NotesThe plaques are clear and approximately 2 millimeters in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Aziz_1

Aziz_2

Aziz_3

Aziz_4
(Lsr2-like DNA bridging protein)

Aziz_5

Aziz_6

Aziz_7

Aziz_8

Aziz_9

Aziz_10

Aziz_11

Aziz_12

Aziz_13

Aziz_14

Aziz_15

Aziz_16

Aziz_17
(terminase small subunit)

Aziz_18
(terminase large subunit)

Aziz_19
(portal protein)

Aziz_20
(capsid maturation protease)

Aziz_21
(scaffolding protein)

Aziz_22
(major capsid protein)

Aziz_23
(head-to-tail adaptor)

Aziz_24
(head-to-tail stopper)

Aziz_25

Aziz_26
(tail terminator)

Aziz_27
(major tail protein)

Aziz_28
(tail assembly chaperone)

Aziz_29
(tail assembly chaperone)

Aziz_30
(tape measure protein)

Aziz_31
(minor tail protein)

Aziz_32
(minor tail protein)

Aziz_33
(minor tail protein)

Aziz_34
(minor tail protein)

Aziz_35
(minor tail protein)

Aziz_36

Aziz_37

Aziz_38

Aziz_39

Aziz_40
(lysin A)

Aziz_41

Aziz_42
(holin)

Aziz_43
(lysin B)

Aziz_44
(NrdH-like glutaredoxin)

Aziz_45

Aziz_46

Aziz_47

Aziz_48

Aziz_49

Aziz_50

Aziz_51

Aziz_52

Aziz_53

Aziz_54
(nucleotidyl transferase)

Aziz_55

Aziz_56

Aziz_57

Aziz_58

Aziz_59

Aziz_60
(ERF family ssDNA binding protein)

Aziz_61

Aziz_62
(exonuclease)

Aziz_63

Aziz_64
(ClpP-like protease)

Aziz_65

Aziz_66

Aziz_67

Aziz_68

Aziz_69

Aziz_70

Aziz_71

Aziz_72

Aziz_73

Aziz_74
(ssDNA-binding protein)

Aziz_75

Aziz_76
(DnaB-like dsDNA helicase)

Aziz_77
(WhiB family transcription factor)

Aziz_78
(holliday junction resolvase)

Aziz_79
(WhiB family transcription factor)

Aziz_80

Aziz_81

Aziz_82

Aziz_83

Aziz_84

Aziz_85

Aziz_86

Aziz_87
(DNA polymerase III subunit)

Aziz_88

Aziz_89

Aziz_90

Aziz_91

Aziz_92
(HNH endonuclease)

Aziz_93

Aziz_94

Aziz_95

Aziz_96

Aziz_97

Aziz_98

Aziz_99

Aziz_100
(HNH endonuclease)

Aziz_101

Aziz_105

Aziz_110

Aziz_113

Aziz_117

Aziz_128

Aziz_129

Aziz_130
(RtcB-like RNA ligase)

Aziz_131

Aziz_132
(PTPc tyrosine phosphatase)

Aziz_133

Aziz_134

Aziz_135

Aziz_136

Aziz_137

Aziz_138

Aziz_139
(DprA-like DNA processing chain A)

Aziz_140

Aziz_141
(metallophosphoesterase)

Aziz_142

Aziz_143

Aziz_144

Aziz_145
(serine integrase)

Aziz_146

Aziz_147

Aziz_148

Aziz_149

Aziz_150

Aziz_151

Aziz_152

Aziz_153

Aziz_154

Aziz_155

Aziz_156

Aziz_157

Aziz_158

Aziz_159

Aziz_160

Aziz_161

Aziz_162

Aziz_163

Aziz_164

Aziz_165

Aziz_166

Aziz_167

Aziz_168

Aziz_169

Aziz_170

Aziz_171

Aziz_172

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT658802
Refseq NumberNone yet
Published in PaperFitzgerald et al 2021, Microbiology Resource Announcements: Genome Sequences of Subcluster M2 Mycobacteriophages Estes and Aziz
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.19x10^8
Date of SEA Lysate Titering Apr 26, 2017
Pitt Freezer Box# 64
Pitt Freezer Box Grid# A9
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload