Mycobacterium phage Glaske16
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Detailed Information for Phage Glaske16
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesJAST16
Found ByS.Mendez, T.Feroz, A.Ornn, J.Hudson
Year Found2022
Location FoundLongview, TX United States
Finding InstitutionLeTourneau University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates32.465 N, 94.727778 W Map
Discovery NotesThe soil sample was collected at LeTourneau University in Longview, Texas, USA outside of the 53,000-square-foot Glaske Center for Engineering, Science, and Technology building on 23rd August 2022 around 5:00 p.m. CST. The ambient temperature was 28.3 °C and the weather was mostly cloudy. The soil was moist because it had rained the night before, about 2.05 in.
Naming NotesThe former name of our phage was JAST16, that was derived by taking the first name initials of each member of our research team, and 16 for our sample number allocated by the instructor during the enrichment isolation process of various phages. However, the “JAST16” name was not accepted, so we changed it to Glaske16. Since the sample was collected in front of the Glaske building and 16 was our sample number, we decided to rename it as Glaske16.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 28, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage360
Genome length (bp)81156
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceACCTCCTGCAA
GC Content61.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterM
SubclusterM1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionLeTourneau University
Annotation StatusBeing Annotated (Expected completion by 5/1/2023)
Plaque NotesThe phage exhibited clear plaques with the average diameter of 1.5mm. The average distance between two plaques on the same plate was 1. 5cm.The plaques were generally round and uniform across the entire plate.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 4.9 x 10^9 pfu/ml
Available Files
Plaque PictureDownload
EM PictureDownload