Detailed Information for Phage Rey |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Christina Ferreira |
Year Found | 2008 |
Location Found | Houston (Harris County), TX USA |
Finding Institution | University of Pittsburgh |
Program | Phage Hunters Integrating Research and Education |
From enriched soil sample? | Unknown |
Isolation Temperature | Not entered |
GPS Coordinates | 29.725031 N, 95.28519 W Map |
Growth Notes | In order to recover Rey plaques and make a lysate, it is helpful to use only 250 ul of M. smegmatis culture to make the lawn and incubate plates at room temperature over a few days. (Greg Broussard, 2013.) |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | May 11, 2011 |
Genome length (bp) | 83724 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 11 bases |
Overhang Sequence | ACCCCATGCAA |
GC Content | 60.9% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | M |
Subcluster | M2 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Morphotype | Siphoviridae |
Number of Genes | 153 |
Number of tRNAs | 21 |
Number of tmRNAs | 1 |
Has been Phamerated? | Yes |
Gene List |
|
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | JF937105 |
Refseq Number | None yet |
Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
2 |
Pitt Freezer Box Grid# |
H1 |
Available Files |
Plaque Picture | Download |
Virtual Digest Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |