Mycobacterium phage Rey
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Detailed Information for Phage Rey
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByChristina Ferreira
Year Found2008
Location FoundHouston (Harris County), TX USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates29.725031 N, 95.28519 W Map
Growth NotesIn order to recover Rey plaques and make a lysate, it is helpful to use only 250 ul of M. smegmatis culture to make the lawn and incubate plates at room temperature over a few days. (Greg Broussard, 2013.)
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 11, 2011
Genome length (bp)83724
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceACCCCATGCAA
GC Content60.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterM
SubclusterM2
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes153
Number of tRNAs21
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Rey_1

Rey_2

Rey_3
(Lsr2-like DNA bridging protein)

Rey_4

Rey_5

Rey_6

Rey_7

Rey_8

Rey_9

Rey_10

Rey_11

Rey_12

Rey_13

Rey_14

Rey_15

Rey_16

Rey_17

Rey_18
(terminase large subunit)

Rey_19
(portal protein)

Rey_20
(MuF-like minor capsid protein)

Rey_21
(scaffolding protein)

Rey_22
(major capsid protein)

Rey_23
(head-to-tail adaptor)

Rey_24
(head-to-tail stopper)

Rey_25

Rey_26
(tail terminator)

Rey_27
(major tail protein)

Rey_29
(tail assembly chaperone)

Rey_28
(tail assembly chaperone)

Rey_30
(tape measure protein)

Rey_31
(minor tail protein)

Rey_32
(minor tail protein)

Rey_33
(minor tail protein)

Rey_34
(minor tail protein)

Rey_35
(minor tail protein)

Rey_36

Rey_37

Rey_38

Rey_39

Rey_40
(lysin A)

Rey_41

Rey_42
(holin)

Rey_43
(lysin B)

Rey_44
(NrdH-like glutaredoxin)

Rey_45

Rey_46

Rey_47

Rey_48

Rey_49

Rey_50

Rey_51

Rey_52

Rey_53

Rey_54

Rey_55
(nucleotidyltransferase)

Rey_56

Rey_57

Rey_58

Rey_59

Rey_60

Rey_61
(ERF family ssDNA binding protein)

Rey_62

Rey_63

Rey_64

Rey_65
(ClpP-like protease)

Rey_66

Rey_67

Rey_68

Rey_69

Rey_70

Rey_71

Rey_72

Rey_73
(ssDNA binding protein)

Rey_74

Rey_75
(DnaB-like dsDNA helicase)

Rey_76

Rey_77

Rey_78

Rey_79
(WhiB family transcription factor)

Rey_80

Rey_81

Rey_82

Rey_83

Rey_84

Rey_85

Rey_86
(DnaQ-like DNA polymerase III subunit)

Rey_87

Rey_88

Rey_89

Rey_90

Rey_91

Rey_92

Rey_93

Rey_94

Rey_95

Rey_96

Rey_97

Rey_98

Rey_99

Rey_100

Rey_101
(HNH endonuclease)

Rey_102

Rey_103

Rey_107

Rey_113

Rey_114

Rey_117

Rey_121

Rey_131

Rey_132

Rey_133

Rey_134
(RtcB-like RNA ligase)

Rey_135
(PTPc tyrosine phosphatase)

Rey_136

Rey_137

Rey_138

Rey_139

Rey_140

Rey_141

Rey_142
(DprA-like DNA processing chain A)

Rey_143

Rey_144
(metallophosphoesterase)

Rey_145

Rey_146

Rey_147

Rey_148
(serine integrase)

Rey_149

Rey_150

Rey_151

Rey_152

Rey_153

Rey_154

Rey_155

Rey_156

Rey_157

Rey_158

Rey_159

Rey_160

Rey_161

Rey_162

Rey_163

Rey_164

Rey_165

Rey_166

Rey_167

Rey_168

Rey_169

Rey_170

Rey_171

Rey_172

Rey_173

Rey_174

Rey_175

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionJF937105
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 2
Pitt Freezer Box Grid# H1
Available Files
Plaque PictureDownload
Virtual Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload