Mycobacterium phage GardenSalsa
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Detailed Information for Phage GardenSalsa
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySam McKee
Year Found2015
Location FoundPittsburgh, PA USA
Finding InstitutionSeton Hill University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.337159 N, 80.081037 W Map
Discovery NotesThis phage was isolated from a soil sample obtained from a compost, mulch pile near a vegetable garden on 8/29/2015 at 18:00. The air temperature was 82°F. The sample was taken about 1 foot into the pile at about 3 inches in depth. The soil was light brown in color and appeared very dry. There were signs of biological life in the area and there was surrounding vegetation like decomposing grass and vegetables. The sample site was approximately 6 feet from the base of a maple tree.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 12, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage343
Genome length (bp)80309
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceACCCCATGCAA
GC Content60.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterM
SubclusterM2
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesThe plaque appeared to be turbid and cloudy and is about 0.5 mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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GardenSalsa_1

GardenSalsa_2

GardenSalsa_3

GardenSalsa_4
(Lsr2-like DNA bridging protein)

GardenSalsa_5

GardenSalsa_6

GardenSalsa_7

GardenSalsa_8

GardenSalsa_9

GardenSalsa_10

GardenSalsa_11

GardenSalsa_12

GardenSalsa_13

GardenSalsa_14

GardenSalsa_15

GardenSalsa_16

GardenSalsa_17
(terminase small subunit)

GardenSalsa_18
(terminase large subunit)

GardenSalsa_19
(portal protein)

GardenSalsa_20
(MuF-like minor capsid protein)

GardenSalsa_21
(scaffolding protein)

GardenSalsa_22
(major capsid protein)

GardenSalsa_23
(head-to-tail adaptor)

GardenSalsa_24
(head-to-tail stopper)

GardenSalsa_25

GardenSalsa_26
(tail terminator)

GardenSalsa_27
(major tail protein)

GardenSalsa_28
(tail assembly chaperone)

GardenSalsa_29
(tail assembly chaperone)

GardenSalsa_30
(tape measure protein)

GardenSalsa_31
(minor tail protein)

GardenSalsa_32
(minor tail protein)

GardenSalsa_33
(minor tail protein)

GardenSalsa_34
(minor tail protein)

GardenSalsa_35
(minor tail protein)

GardenSalsa_36
(minor tail protein)

GardenSalsa_37

GardenSalsa_38

GardenSalsa_39

GardenSalsa_40
(lysin A)

GardenSalsa_41

GardenSalsa_42
(holin)

GardenSalsa_43
(lysin B)

GardenSalsa_44
(NrdH-like glutaredoxin)

GardenSalsa_45

GardenSalsa_46

GardenSalsa_47

GardenSalsa_48

GardenSalsa_49

GardenSalsa_50

GardenSalsa_51

GardenSalsa_52

GardenSalsa_53

GardenSalsa_54

GardenSalsa_55

GardenSalsa_56
(nucleotidyltransferase)

GardenSalsa_57

GardenSalsa_58

GardenSalsa_59

GardenSalsa_60

GardenSalsa_61

GardenSalsa_62
(ERF family ssDNA binding protein)

GardenSalsa_63

GardenSalsa_64

GardenSalsa_65

GardenSalsa_66
(ClpP-like protease)

GardenSalsa_67

GardenSalsa_68

GardenSalsa_69

GardenSalsa_70

GardenSalsa_71

GardenSalsa_72

GardenSalsa_73

GardenSalsa_74

GardenSalsa_75

GardenSalsa_76
(ssDNA binding protein)

GardenSalsa_77

GardenSalsa_78
(DnaB-like dsDNA helicase)

GardenSalsa_79

GardenSalsa_80
(holliday junction resolvase)

GardenSalsa_81
(WhiB family transcription factor)

GardenSalsa_82

GardenSalsa_83

GardenSalsa_84

GardenSalsa_85

GardenSalsa_86

GardenSalsa_87

GardenSalsa_88

GardenSalsa_89

GardenSalsa_90
(DnaQ-like DNA polymerase III subunit)

GardenSalsa_91

GardenSalsa_92

GardenSalsa_93

GardenSalsa_94
(HNH endonuclease)

GardenSalsa_95

GardenSalsa_96

GardenSalsa_97

GardenSalsa_98

GardenSalsa_99

GardenSalsa_100
(HNH endonuclease)

GardenSalsa_101

GardenSalsa_102

GardenSalsa_110

GardenSalsa_113

GardenSalsa_117

GardenSalsa_128

GardenSalsa_129

GardenSalsa_130
(RtcB-like RNA ligase)

GardenSalsa_131

GardenSalsa_132
(PTPc tyrosine phosphatase)

GardenSalsa_133

GardenSalsa_134

GardenSalsa_135

GardenSalsa_136

GardenSalsa_137

GardenSalsa_138

GardenSalsa_139

GardenSalsa_140
(DprA-like DNA processing chain A)

GardenSalsa_141

GardenSalsa_142
(metallophosphoesterase)

GardenSalsa_143

GardenSalsa_144

GardenSalsa_145

GardenSalsa_146
(serine integrase)

GardenSalsa_147

GardenSalsa_148

GardenSalsa_149

GardenSalsa_150

GardenSalsa_151

GardenSalsa_152

GardenSalsa_153

GardenSalsa_154

GardenSalsa_155

GardenSalsa_156

GardenSalsa_157

GardenSalsa_158

GardenSalsa_159

GardenSalsa_160

GardenSalsa_161

GardenSalsa_162

GardenSalsa_163

GardenSalsa_164

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKY783914
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 21
Pitt Freezer Box Grid# G8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload