| Detailed Information for Phage Aziz |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Trevor Ruesch and Carol Stepniewski |
| Year Found | 2017 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 47.667416 N, 117.401525 W Map |
| Discovery Notes | The isolation was enriched and was conducted at a room temperature of 22 degree C. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 7, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 749 |
| Genome length (bp) | 83412 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACCCCATGCAA |
| GC Content | 60.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | M |
| Subcluster | M2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Gonzaga University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are clear and approximately 2 millimeters in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 150 |
| Number of tRNAs | 21 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT658802 |
| Refseq Number | None yet |
| Published in Paper | Fitzgerald et al 2021, Microbiology Resource Announcements: Genome Sequences of Subcluster M2 Mycobacteriophages Estes and Aziz |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.19x10^8 |
| Date of SEA Lysate Titering |
Apr 26, 2017 |
| Pitt Freezer Box# |
64 |
| Pitt Freezer Box Grid# |
A9 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
