Mycobacterium phage Bongo
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Detailed Information for Phage Bongo
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByHan Yuan and James Wherley
Year Found2009
Location FoundSt Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.644208 N, 90.314927 W Map
Discovery NotesWashington University Campus under a tree near basketball courts. Soil depth: 3cm. Temperature 24 degree Celsius.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 23, 2011
Genome length (bp)80228
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceACCTCCTGCAA
GC Content61.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterM
SubclusterM1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notes1mm/ clear/ Incubation 24-48 hours at 37 degrees Celsius.
MorphotypeSiphoviridae
Number of Genes132
Number of tRNAs20
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Bongo_1

Bongo_2

Bongo_3
(Lsr2-like DNA bridging protein)

Bongo_4

Bongo_5

Bongo_6

Bongo_7

Bongo_8

Bongo_9

Bongo_10

Bongo_11

Bongo_12

Bongo_13

Bongo_14
(terminase large subunit)

Bongo_15
(portal protein)

Bongo_16
(MuF-like minor capsid protein)

Bongo_17
(scaffolding protein)

Bongo_18
(major capsid protein)

Bongo_19
(head-to-tail adaptor)

Bongo_20
(head-to-tail stopper)

Bongo_21

Bongo_22
(tail terminator)

Bongo_23
(major tail protein)

Bongo_25
(tail assembly chaperone)

Bongo_24
(tail assembly chaperone)

Bongo_26
(tape measure protein)

Bongo_27
(minor tail protein)

Bongo_28
(minor tail protein)

Bongo_29
(minor tail protein)

Bongo_30
(minor tail protein)

Bongo_31

Bongo_32

Bongo_33

Bongo_34

Bongo_35
(lysin A)

Bongo_36

Bongo_37
(holin)

Bongo_38
(lysin B)

Bongo_39
(NrdH-like glutaredoxin)

Bongo_40

Bongo_41

Bongo_42

Bongo_43

Bongo_44

Bongo_45

Bongo_46

Bongo_47
(nucleotidyl transferase)

Bongo_48

Bongo_49

Bongo_50

Bongo_51

Bongo_52

Bongo_53

Bongo_54
(ERF family ssDNA binding protein)

Bongo_55

Bongo_56

Bongo_57

Bongo_58
(ClpP-like protease)

Bongo_59

Bongo_60

Bongo_61

Bongo_62

Bongo_63

Bongo_64

Bongo_65

Bongo_66

Bongo_67
(ssDNA binding protein)

Bongo_68

Bongo_69
(DnaB-like dsDNA helicase)

Bongo_70

Bongo_71

Bongo_72
(WhiB family transcription factor)

Bongo_73

Bongo_74

Bongo_75

Bongo_76

Bongo_77
(endonuclease VII)

Bongo_78

Bongo_79
(DnaQ-like DNA polymerase III subunit)

Bongo_80

Bongo_81

Bongo_82

Bongo_83
(PTPc tyrosine phosphatase)

Bongo_84

Bongo_85

Bongo_86

Bongo_87

Bongo_88

Bongo_89
(HNH endonuclease)

Bongo_90

Bongo_91 — tRNA
(gp91; tRNA-Trp(cca))

Bongo_92 — tmRNA
(tmRNA)

Bongo_93 — tRNA
(tRNA-Asn(gtt))

Bongo_94 — tRNA
(gp94; tRNA-Arg(acg))

Bongo_95 — tRNA
(gp95; tRNA-Tyr(gta))

Bongo_96 — tRNA
(gp96; tRNA-Gln(ctg))

Bongo_97 — tRNA
(gp97; tRNA-Pro(tgg))

Bongo_98 — tRNA
(gp98; tRNA-Ser(gct))

Bongo_99 — tRNA
(gp99; tRNA-Phe(gaa))

Bongo_100 — tRNA
(gp100; tRNA-Met(cat))

Bongo_101

Bongo_102 — tRNA
(gp102; tRNA-Arg(acg))

Bongo_103 — tRNA
(gp103; tRNA-His(gtg))

Bongo_104 — tRNA
(gp104; tRNA-Cys(gca))

Bongo_105

Bongo_106 — tRNA
(gp106; tRNA-Leu(cag))

Bongo_107
(ribonucleotide reductase)

Bongo_108 — tRNA
(gp108; tRNA-Lys(ctt))

Bongo_109 — tRNA
(gp109; tRNA-Ile(gat))

Bongo_110 — tRNA
(gp110; tRNA-Gly(tcc))

Bongo_111 — tRNA
(gp111; tRNA-Val(tac))

Bongo_112 — tRNA
(gp112; tRNA-Thr(agt))

Bongo_113 — tRNA
(gp113; tRNA-Asp(gtc))

Bongo_114 — tRNA
(gp114; tRNA-Glu(ctc))

Bongo_115
(RtcB-like RNA ligase)

Bongo_116
(band-7-like membrane protein)

Bongo_117
(MazG-like nucleotide pyrophosphohydrolase)

Bongo_118

Bongo_119

Bongo_120

Bongo_121

Bongo_122

Bongo_123
(DprA-like DNA processing chain A)

Bongo_124

Bongo_125
(metallophosphoesterase)

Bongo_126

Bongo_127

Bongo_128

Bongo_129

Bongo_130
(serine integrase)

Bongo_131

Bongo_132

Bongo_133

Bongo_134

Bongo_135

Bongo_136

Bongo_137

Bongo_138

Bongo_139

Bongo_140

Bongo_141

Bongo_142

Bongo_143

Bongo_144

Bongo_145

Bongo_146

Bongo_147

Bongo_148

Bongo_149

Bongo_150

Bongo_151

Bongo_152

Bongo_153

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionJN699628
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator WUSL
SEA Lysate Titer 8.4e8
Pitt Freezer Box# 1
Pitt Freezer Box Grid# C7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload