| Detailed Information for Phage CassieYates |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Cassandra Quainoo |
| Year Found | 2021 |
| Location Found | Blacksburg, VA United States |
| Finding Institution | Virginia Tech |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.225 N, 80.42 W Map |
| Discovery Notes | My phage was found in an enriched soil sample near the Prairie Quad next to the East wing of Peddrew-Yates residential hall on Virginia Tech's campus. |
| Naming Notes | I chose the name of this phage because I used to reside at Peddrew-Yates, which is a living-learning community here at Virginia Tech that emphasizes the college experience for their African American students. Cassie is short for my first name and Yates for the first African American graduate at Virginia Tech. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 13, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1079 |
| Genome length (bp) | 41901 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCCATGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | G |
| Subcluster | G1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Virginia Tech |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were turbid and had a cloudy hue to them. Some plaques also seemed to overlap each other. |
| Morphotype | Siphoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON392160 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
134 |
| Pitt Freezer Box Grid# |
A10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
