| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | F. W. Baliraine, T. Pledger, C. Andrade, C. Wade |
| Year Found | 2022 |
| Location Found | Longview, TX United States |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.46474 N, 94.7272 W Map |
| Discovery Notes | The soil sample was collected from a landscaping bush outside of the Glaske Center for Engineering, Science, and Technology building at LeTourneau University at 10:18 a.m. CST on August 23, 2022. The weather was very cloudy and humid with a 90% chance of rain and an ambient temperature of 23.9°C. The soil was very damp and muddy at the surface since it had been raining for the past few days, with 2.2 inches in the last 24 hours. There was plant debris covering the surface. We dug approximately 2 inches below the plant debris. The soil was rich and dark with worms, grubs, and other organisms present, but we made sure that no worms or grubs were present in the soil sample that we took to the lab for further processing. |
| Naming Notes | Our lab group isolated two phages, which is where the "di-" prefix originates from. Notably, the two phages showed drastically different plague sizes. Our other phage, Dimaximus, created significantly larger plaques compared to this phage. In Latin, “maximus” refers to the "greatest" or "largest," whereas “minimus” refers to the "smallest, smaller, or least." Since this phage creates very small plaques, we decided to name it “Diminimus.” |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 28, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 338 |
| Genome length (bp) | 80037 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACCTCCTGCAA |
| GC Content | 61.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | M |
| Subcluster | M1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | LeTourneau University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques of Diminimus looked a bit clear, glossy, and very small in size, with an average diameter of 0.8 mm (0.4-1.1; n = 15). They were very consistent in size and appearance across our many plates. |
| Morphotype | Siphoviridae |
| Number of Genes | 137 |
| Number of tRNAs | 19 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR521083 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
150 |
| Pitt Freezer Box Grid# |
D9 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
